Linked Life Data

8229936

Source:http://linkedlifedata.com/resource/pubmed/id/8229936

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1993-12-21
pubmed:abstractText
Dog spermatozoa from fresh ejaculates and after freezing-thawing were air-dried on to grids and subjected to X-ray microanalysis or examined by scanning and transmission electron microscopy. The spermatozoa subjected to freezing-thawing presented major changes in their morphology, which included the loss of acrosomal contents, seen both by the swelling and rarefaction of the acrosome and the loss of electron-dense acrosomal material. In many cells the acrosomal damage, including the equatorial region, was conspicuous, with vesiculization of the acrosomal membranes. The plasmalemma remained, in most cases, apparently intact. X-ray microanalysis of the sperm aliquots showed a significant decrease in the amount of most selected elements after freezing-thawing. The extent to which concentrations of phosphorus and sulfur decreased in the sperm head region suggested that major changes in the chromatin occurred during the freeze-thaw process.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0449-3087
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
279-85
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Fine structure and elemental composition of fresh and frozen dog spermatozoa.
pubmed:affiliation
Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't