Linked Life Data

8228868

Source:http://linkedlifedata.com/resource/pubmed/id/8228868

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-12-21
pubmed:abstractText
The two cell populations of the day 5 mouse blastocyst--the inner cell mass (ICM) and trophectoderm (TE)--were isolated by immunosurgery and TE biopsy. The uptake of glucose and pyruvate and the production of lactate were measured in the isolated cell populations and in single day 5 blastocysts, using a non-invasive ultramicrofluorescence technique. The number of cells in isolated ICMs, TE vesicles, and intact blastocysts were counted after differential labeling with fluorochromes. The uptake of glucose and the formation of lactate were approximately three and five times greater, respectively, in the ICM cells compared with the TE cells. All the glucose consumed by the ICM could be accounted for by lactate formation, whereas the value for TE was 55%. The uptake of pyruvate was low in both cell types. The results are consistent with the proposition that the TE acts as a transporting epithelium, sparing nutrients for metabolism by the ICM.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-104X
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
267
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
337-43
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Energy metabolism of the trophectoderm and inner cell mass of the mouse blastocyst.
pubmed:affiliation
Department of Biology, University of York, Heslington, England.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't