Linked Life Data

8226960

Source:http://linkedlifedata.com/resource/pubmed/id/8226960

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
32
pubmed:dateCreated
1993-12-13
pubmed:abstractText
This study was undertaken to examine the regulation of renin release and gene expression in primary cultures of juxtaglomerular granular (JGG) cells. JGG cells, isolated from mouse kidney, demonstrated high purity and showed regulated renin release in vitro. Changes in steady-state renin mRNA levels were assessed by quantitative polymerase chain reaction techniques, with polymerase chain reaction amplification efficiency monitored by co-amplification of experimental samples with a dilution series of cDNA for a mutant template. When the cells were incubated in the presence or absence of forskolin, isoproterenol, or 8-bromo-cAMP plus 3-isobutyl-1-methylxanthine for 24 h or cholera toxin for 12 h, renin mRNA levels were increased 3.9-, 4.4-, 5.1-, and 3.3-fold, respectively (all, p < 0.05). A significant increase in renin mRNA levels was observed 8 h after treatment with forskolin, but no change was detectable at 4 h. Cycloheximide did not prevent the increase in renin mRNA by isoproterenol. When RNA synthesis was inhibited by incubation with actinomycin D (5 micrograms/ml), renin mRNA levels declined with a half-life of 3.0 +/- 0.8 h. Treatment with forskolin increased renin mRNA half-life to 10.8 +/- 2.7 h (p < 0.025). The half-life of beta-actin, endothelin-1, or the facilitative glucose transporter-1 (GLUT-1) mRNA expressed in the same cells was not altered, although the steady-state levels of GLUT-1 mRNA increased 2.2-fold after treatment with forskolin. These data demonstrate that cAMP increases renin release and mRNA levels in JGG cells in vitro, that the stimulatory effect of cAMP on renin mRNA is delayed but does not require new protein synthesis, and that the increased renin mRNA levels induced by cAMP are due in part to a selective increase in renin mRNA stability.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
268
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
24138-44
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8226960-8-Bromo Cyclic Adenosine Monophosphate, pubmed-meshheading:8226960-Animals, pubmed-meshheading:8226960-Base Sequence, pubmed-meshheading:8226960-Cells, Cultured, pubmed-meshheading:8226960-Cholera Toxin, pubmed-meshheading:8226960-Cyclic AMP, pubmed-meshheading:8226960-Cycloheximide, pubmed-meshheading:8226960-DNA, Complementary, pubmed-meshheading:8226960-Dactinomycin, pubmed-meshheading:8226960-Isoproterenol, pubmed-meshheading:8226960-Juxtaglomerular Apparatus, pubmed-meshheading:8226960-Male, pubmed-meshheading:8226960-Mice, pubmed-meshheading:8226960-Mice, Inbred C57BL, pubmed-meshheading:8226960-Molecular Sequence Data, pubmed-meshheading:8226960-Polymerase Chain Reaction, pubmed-meshheading:8226960-RNA, Messenger, pubmed-meshheading:8226960-Rats, pubmed-meshheading:8226960-Renin
pubmed:year
1993
pubmed:articleTitle
Cyclic AMP selectively increases renin mRNA stability in cultured juxtaglomerular granular cells.
pubmed:affiliation
Department of Physiology, University of Michigan, Ann Arbor 48109.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.