Linked Life Data

8224542

Source:http://linkedlifedata.com/resource/pubmed/id/8224542

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-12-16
pubmed:databankReference
pubmed:abstractText
The gene con-6 of Neurospora crassa is expressed during the formation of asexual spores (conidia), but it is not expressed in mycelium. con-6 mRNA appears upon induction of conidiation and reaches high levels at the late stages of conidiation, and in mature conidia. The CON6 polypeptide and a CON6-beta-Gal fusion protein were present at high levels only in free conidia. Shortly after spore germination con-6 mRNA disappears and the CON6 polypeptide is degraded. CON6 is a small, hydrophilic polypeptide containing a repeat sequence; it not homologous to any known protein but has features resembling the late embryogenesis abundant proteins of maize. Inactivation of con-6 by the repeat-induced point mutation process had no demonstrable effect on formation or germination of conidia. Upstream sequence comparisons for con-6 and other con genes identified a common potential regulatory sequence, designated CRS-B. DNA mobility shift analyses with cell extracts identified a factor that bound to synthetic DNA fragments containing this sequence. This binding factor was present in mycelium but not in conidiating cultures. Experiments with independent integrated con-6'-'lacZ translational fusions revealed substantial variability of expression among transformants carrying identical fusion constructs: This variability may be due to the differential methylation of transformant DNA noted by others.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0012-1606
pubmed:author
pubmed:issnType
Print
pubmed:volume
160
pubmed:geneSymbol
con-6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
254-64
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Structural characterization and expression analysis of the Neurospora conidiation gene con-6.
pubmed:affiliation
Department of Biological Sciences, Stanford University, California 94305.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't