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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1993-12-15
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pubmed:abstractText |
Cultured human muscle cells were depleted of mitochondrial DNA (mtDNA) by prolonged treatment with ethidium bromide (EB). In these respiration-deficient muscle cells neither cytochrome c oxidase activity nor mtDNA were detectable. However, mitochondrial matrix enzymes remained present and were localized in mitochondria-like organelles, as shown by subcellular fractionation. Metabolic labeling showed synthesis of cytochrome c oxidase subunits coded by nuclear DNA (nDNA). These results indicate that depletion of mtDNA in cultured human myoblasts does not inhibit expression of nDNA-coded mitochondrial proteins. The characteristic thread-like pattern of mitochondria was lost in mtDNA-depleted myoblasts, as shown by immunofluorescence with antibodies against cytochrome c oxidase and the F1 part of the mitochondrial ATP synthase (F1-ATPase) and by fluorescence of the carbocyanine dye, 3,3'-dipentyloxacarbocyanine iodide (DiOC5(3)). The organelles visualized by these methods were round and swollen and had a localization different from lysosomes as shown by double-labeling with mitochondrial and lysosomal antibodies. These results indicate that not only synthesis, but also import of mitochondrial proteins into mitochondria-like organelles remains possible in respiration-deficient cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0171-9335
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
61
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
400-8
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8223726-Cell Nucleus,
pubmed-meshheading:8223726-Citrate (si)-Synthase,
pubmed-meshheading:8223726-DNA, Mitochondrial,
pubmed-meshheading:8223726-DNA Damage,
pubmed-meshheading:8223726-Electron Transport,
pubmed-meshheading:8223726-Electron Transport Complex IV,
pubmed-meshheading:8223726-Ethidium,
pubmed-meshheading:8223726-Gene Expression Regulation,
pubmed-meshheading:8223726-Glutamate Dehydrogenase,
pubmed-meshheading:8223726-Humans,
pubmed-meshheading:8223726-L-Lactate Dehydrogenase,
pubmed-meshheading:8223726-Mitochondria, Muscle,
pubmed-meshheading:8223726-Muscle Proteins,
pubmed-meshheading:8223726-Oxygen Consumption,
pubmed-meshheading:8223726-Proton-Translocating ATPases,
pubmed-meshheading:8223726-beta-N-Acetylhexosaminidases
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pubmed:year |
1993
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pubmed:articleTitle |
Mitochondria in cultured human muscle cells depleted of mitochondrial DNA.
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pubmed:affiliation |
Department of Neurology, University of Amsterdam, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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