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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
1993-12-17
|
| pubmed:abstractText |
We report the development of the first radioimmunoassay for YKL-40, a M(r) = 40 kDa protein which is secreted at high levels by human synovial cells and articular cartilage chondrocytes, and by the human osteosarcoma cell line MG63. This assay uses YKL-40 purified from the conditioned medium of MG63 cells as standard and tracer, and as antigen for immunizing rabbits. With this assay we have discovered high levels of YKL-40 antigen in serum and SF. The molecular weight of serum and SF YKL-40 is identical to purified YKL-40. To evaluate the possible utility of YKL-40 in the assessment of joint disease, we measured YKL-40 in serum and SF of 49 patients with various forms of inflammatory and degenerative joint disease and in the serum of 50 normal adults. The YKL-40 level in serum was significantly higher (P < 0.001) in the patients compared to the normal adults, but there was no difference in serum YKL-40 between the patients with inflammatory joint diseases and OA. The SF levels of YKL-40 were 15-fold higher than serum levels and there was a significant correlation (r = 0.55, P < 0.001) between YKL-40 concentration in SF and serum. Although the tissue distribution of YKL-40 secretion is presently unknown, these observations suggest that a major portion of serum YKL-40 in fact arises from the joint.(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0263-7103
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
32
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
949-55
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8220933-Adipokines,
pubmed-meshheading:8220933-Adult,
pubmed-meshheading:8220933-Aged,
pubmed-meshheading:8220933-Aged, 80 and over,
pubmed-meshheading:8220933-Amino Acid Sequence,
pubmed-meshheading:8220933-Biological Markers,
pubmed-meshheading:8220933-Female,
pubmed-meshheading:8220933-Glycoproteins,
pubmed-meshheading:8220933-Humans,
pubmed-meshheading:8220933-Joints,
pubmed-meshheading:8220933-Lectins,
pubmed-meshheading:8220933-Male,
pubmed-meshheading:8220933-Middle Aged,
pubmed-meshheading:8220933-Molecular Sequence Data,
pubmed-meshheading:8220933-Proteins,
pubmed-meshheading:8220933-Radioimmunoassay,
pubmed-meshheading:8220933-Rheumatic Diseases,
pubmed-meshheading:8220933-Synovial Fluid,
pubmed-meshheading:8220933-Wounds and Injuries
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
A new biochemical marker for joint injury. Analysis of YKL-40 in serum and synovial fluid.
|
| pubmed:affiliation |
Department of Biology, University of California, San Diego, La Jolla.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|