Linked Life Data

8220480

Source:http://linkedlifedata.com/resource/pubmed/id/8220480

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-12-21
pubmed:abstractText
Pathogenesis-related (PR) proteins form a heterogeneous group of host-encoded, low-molecular-mass proteins that are secreted through the exocytic pathway. They are synthesized by the plant in response to various stimuli, including pathogen attack or exposure to certain chemicals. The PRB-1b gene of Nicotiana tabacum codes for a basic-type PR-1 protein whose transcription is regulated by ethylene. A minimal ethylene-responsive promoter element was defined by deletion analysis in transgenic tobacco plants. Promoter sequences containing 213 bp or more were sufficient to enhance a 20-fold increase of beta-glucuronidase reporter gene expression in transgenic tobacco leaves exposed to 20 microliters l-1 of ethylene, while 67 bp were not sufficient to trigger ethylene responsiveness. All the constructs that retained ethylene inducibility exhibited phloem-specific activity, which was constitutive in petiole and pedicel abscission zones. This functional study was correlated to an in vitro screening of the major nuclear proteins' binding sites present on the promoter. Gel-shift analysis using nuclear extracts from ethylene-treated and non-treated plants revealed five sequence-specific protein-DNA complexes on promoter sequences spanning -863 to -142 bp. Constitutive expression of the basic-type PR-1 genes at the leaf and petiole or flower and pedicel interfaces may represent pre-emption of plant defenses against potential pathogens, suggesting a functional similarity to pathogen-induced expression in the leaf.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0960-7412
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:geneSymbol
PRB-1b
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
225-34
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
A basic-type PR-1 promoter directs ethylene responsiveness, vascular and abscission zone-specific expression.
pubmed:affiliation
Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't