Linked Life Data

8219506

Source:http://linkedlifedata.com/resource/pubmed/id/8219506

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-12-1
pubmed:abstractText
Seventy-nine representative strains of Mycobacterium farcinogenes, Mycobacterium fortuitum, Mycobacterium peregrinum and Mycobacterium senegalense were analysed by thin-layer chromatography for diagnostic glycolipid and mycolic acid patterns. On the basis of glycolipid patterns the 16 M. senegalense strains were assigned to four groups (I, II, III and IV); one strain received as M. farcinogenes had glycolipids that corresponded to those given by the M. senegalense group III strain. Representatives of M. fortuitum produced alkalilabile glycolipids whereas the M. peregrinum strains produced alkali-stable glycopeptidolipids related to those of M. senegalense group I. Members of M. senegalense groups I, III and IV and the M. farcinogenes strains gave the characteristic pattern consisting of alpha- and epoxymycolates on thin-layer chromatographic analysis of the products of alkaline hydrolysis with 5% aqueous tetrabutylammonium hydroxide. Extraction of mycolates from the same strains using acid methanolysis revealed alpha-mycolates and characteristic more polar long-chain components originating from epoxymycolates. In contrast, the M. senegalense group II strains contained an additional more polar omega-1 methoxymycolate that was detected from both acid and alkaline hydrolysates; this spot was also seen in some M. fortuitum and M. peregrinum strains. Preliminary investigations, using a combination of thin-layer chromatography and immunostaining, showed that glycopeptidolipids from M. peregrinum and M. senegalense group I strains were antigenic, a cross-reaction between members of these taxa was shown. In contrast, no reaction was detected between glycopeptidolipids extracted from M. peregrinum and M. senegalense group I strains with antisera from either M. farcinogenes or M. fortuitum.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0934-8840
pubmed:author
pubmed:issnType
Print
pubmed:volume
279
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
354-67
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Thin-layer chromatographic analysis of glycolipids and mycolic acids from Mycobacterium farcinogenes, Mycobacterium senegalense and related taxa.
pubmed:affiliation
Department of Chemistry, The University, Newcastle upon Tyne, U.K.
pubmed:publicationType
Journal Article, Comparative Study