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rdf:type |
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lifeskim:mentions |
umls-concept:C0011900,
umls-concept:C0040715,
umls-concept:C0085669,
umls-concept:C0150369,
umls-concept:C0332466,
umls-concept:C0337112,
umls-concept:C0443286,
umls-concept:C0599718,
umls-concept:C0599813,
umls-concept:C0599893,
umls-concept:C0919528,
umls-concept:C1511790,
umls-concept:C1519595,
umls-concept:C1522702,
umls-concept:C1524075,
umls-concept:C1555029,
umls-concept:C1826604
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pubmed:issue |
10
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pubmed:dateCreated |
1993-12-22
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pubmed:abstractText |
The chromosomal breakpoints of t(4;11) translocation of acute lymphoblastic leukemia (ALL) have been recently identified at molecular level and shown to involve the AF4 (FEL) gene on chromosome 4 and the ALL-1 (MLL, Hrx) gene on chromosome 11. The ALL-1/AF4 fusion gene is transcribed into a chimeric mRNA. Using primer sets derived from ALL-1 and AF4 cDNAs by reverse transcription-polymerase chain reaction (RT-PCR), we were able to amplify the breakpoint sites of the fusion transcript of all 15 ALL cases with karyotypic or molecular evidence of the t(4;11). DNA fragments of different size were obtained as the consequence of different breakpoints on chromosome 11 and the presence of alternative splicing of ALL-1 exon 8. The feasibility of monitoring the residual cells carrying the t(4;11) in 2 ALL patients with different clinical outcome was evaluated. Overall, the presented results provide evidence that RT-PCR can be used as a rapid method for detecting this chromosomal abnormality and following the patient's response to therapy.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
2943-7
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8219184-Adolescent,
pubmed-meshheading:8219184-Adult,
pubmed-meshheading:8219184-Base Sequence,
pubmed-meshheading:8219184-Child,
pubmed-meshheading:8219184-Chromosomes, Human, Pair 11,
pubmed-meshheading:8219184-Chromosomes, Human, Pair 4,
pubmed-meshheading:8219184-DNA-Binding Proteins,
pubmed-meshheading:8219184-Female,
pubmed-meshheading:8219184-Humans,
pubmed-meshheading:8219184-Infant,
pubmed-meshheading:8219184-Infant, Newborn,
pubmed-meshheading:8219184-Male,
pubmed-meshheading:8219184-Middle Aged,
pubmed-meshheading:8219184-Molecular Sequence Data,
pubmed-meshheading:8219184-Myeloid-Lymphoid Leukemia Protein,
pubmed-meshheading:8219184-Oncogenes,
pubmed-meshheading:8219184-Polymerase Chain Reaction,
pubmed-meshheading:8219184-Precursor Cell Lymphoblastic Leukemia-Lymphoma,
pubmed-meshheading:8219184-Proto-Oncogenes,
pubmed-meshheading:8219184-RNA, Messenger,
pubmed-meshheading:8219184-Recombinant Fusion Proteins,
pubmed-meshheading:8219184-Transcription Factors,
pubmed-meshheading:8219184-Translocation, Genetic
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pubmed:year |
1993
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pubmed:articleTitle |
Detection of ALL-1/AF4 fusion transcript by reverse transcription-polymerase chain reaction for diagnosis and monitoring of acute leukemias with the t(4;11) translocation.
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pubmed:affiliation |
Clinical Pediatrica Università di Milano, Ospedale San Gerardo, Monza, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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