Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1993-12-15
|
| pubmed:abstractText |
Interleukin 3 (IL-3) is a cytokine produced by activated T lymphocytes that is best understood as a hematopoietic growth and differentiation factor. Production of IL-3 by other cell types is controversial; while certain transformed non-lymphocyte cell lines can produce IL-3, it is generally assumed that their non-transformed counterparts do not. It has been previously reported that Pam 212, a transformed murine keratinocyte cell line, produces IL-3. In this study we report that IL-3 can also be secreted by normal murine keratinocytes. Using a cell line (FL5.12) which is responsive to IL-3 and not to other keratinocyte derived cytokines, (e.g. GM-CSF, IL-1 and IL-6), we tested conditioned media from cultures of normal neonatal keratinocytes for biologically active IL-3. These media stimulated the proliferation of FL5.12, and the effect could be neutralized by specific antibodies to IL-3. The presence of IL-3 mRNA was demonstrated by polymerase chain reaction (PCR) amplification of reverse transcribed IL-3 mRNA from cultured normal neonatal keratinocytes and confirmed by Southern blot analysis. By similar techniques, IL-3 mRNA could be identified in freshly isolated neonatal epidermis but not dermis. These data indicated that IL-3 is produced by keratinocytes in the skin of normal neonatal mice, raising the likelihood that the neonatal epidermal microenvironment may have hematopoietic or lymphopoietic properties.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-3,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
1043-4666
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
5
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
240-9
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8218937-Animals,
pubmed-meshheading:8218937-Animals, Newborn,
pubmed-meshheading:8218937-Cell Line,
pubmed-meshheading:8218937-Cell Line, Transformed,
pubmed-meshheading:8218937-Cells, Cultured,
pubmed-meshheading:8218937-Culture Media, Conditioned,
pubmed-meshheading:8218937-Culture Techniques,
pubmed-meshheading:8218937-Cytokines,
pubmed-meshheading:8218937-DNA, Complementary,
pubmed-meshheading:8218937-Electrophoresis, Agar Gel,
pubmed-meshheading:8218937-Epidermis,
pubmed-meshheading:8218937-Gene Expression,
pubmed-meshheading:8218937-Interleukin-2,
pubmed-meshheading:8218937-Interleukin-3,
pubmed-meshheading:8218937-Interleukin-4,
pubmed-meshheading:8218937-Keratinocytes,
pubmed-meshheading:8218937-Mice,
pubmed-meshheading:8218937-Mice, Inbred BALB C,
pubmed-meshheading:8218937-Mice, Inbred Strains,
pubmed-meshheading:8218937-Polymerase Chain Reaction,
pubmed-meshheading:8218937-RNA, Messenger,
pubmed-meshheading:8218937-Recombinant Proteins,
pubmed-meshheading:8218937-Tumor Cells, Cultured
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Production of IL-3 by non-transformed primary neonatal murine keratinocytes: evidence for constitutive IL-3 gene expression in neonatal epidermis.
|
| pubmed:affiliation |
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|