Linked Life Data

8218238

Source:http://linkedlifedata.com/resource/pubmed/id/8218238

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
43
pubmed:dateCreated
1993-11-29
pubmed:abstractText
Galactosylceramides (GalCer) with homogeneous acyl chains containing zero, one, or two cis double bonds have been synthesized and characterized at an argon-aqueous buffer interface using a Langmuir film balance. Both surface pressure and surface potential were measured as a function of molecular area at 24 degrees C. N-Lignoceroylgalactosylsphingosine (N-24:0-GalSph), N-stearoylgalactosylsphingosine (N-18:0-GalSph), and N-palmitoylgalactosylsphingosine (N-16:0-GalSph) form condensed films that are similar to that of bovine brain GalCer, which contains long saturated and mono-unsaturated acyl chains, almost half being hydroxylated. In contrast, a bovine brain GalCer subfraction (NFA-GalCer) that is devoid of the hydroxylated acyl chains displays an apparent two-dimensional phase transition near 9.0 mN/m at 54 A2/molecule. To determine the role of acyl unsaturation in regulating NFA-GalCer's surface behavior, GalCer derivatives containing different mono-unsaturated acyl residues were investigated. N-Nervonoyl-galactosylsphingosine (N-24:1 delta 15-GalSph) and N-docosenoylgalactosylsphingosine (N-22:1 delta 13-GalSph) show liquid-expanded to -condensed phase transitions in their force-area isotherms at 10 and 35 mN/m, respectively. Introduction of acyl chains that are short and saturated [e.g., N-decanoylgalactosylsphingosine (N-10:0-GalSph)] or that are long but contain two cis double bonds [e.g., N-linoleoylgalactosylsphingosine (N-18:2 delta 9,12-GalSph)] causes GalCer to display only liquid-expanded behavior at 24 degrees C. The surface potentials (delta V) of the condensed GalCer derivatives with long saturated acyl residues were quite similar and were over 100 mV higher than that of bovine brain GalCer.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11696-703
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Acyl structure regulates galactosylceramide's interfacial interactions.
pubmed:affiliation
Hormel Institute, University of Minnesota, Austin 55912.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't