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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1993-11-15
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pubmed:abstractText |
alpha 2(I) collagen gene expression is induced in quail embryo chondrocytes pretreated with retinoic acid (RA). The initial appearance of alpha 2(I) mRNA occurs around day 3 of culture in RA-free medium and rapidly progresses over the next 4 days. In transient transfection assays, expression of COL1A2-CAT, a chimeric gene bearing 3500 bp upstream the bone/tendon transcription start site from the human alpha 2(I) gene fused to the CAT gene, is stimulated severalfold in RA-treated chondrocytes. In contrast, enzyme activity is very low in untreated chondrocytes, suggesting that the sequences required for RA-induced transcription of the alpha 2(I) gene are present in this plasmid. Analysis of alpha 2(I) promoter sequences performed with deletion mutants gives overlapping results in collagen type I-producing fibroblasts and chondrocytes withdrawn from RA treatment. These experiments suggest that RA-induced transcription of the alpha 2(I) collagen gene in chondrocytes is regulated by the binding of transcription factors to the same regulatory sequences that control transcription in fibroblasts.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-1358181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-1661245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-1688851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-1717939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-1885609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-2039468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-2055274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-2124188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-2180769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-2197991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-2376598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-2444591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-2553220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-2579081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-2686112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-2857713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-2986135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-2987670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-2987824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-3276711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-3653521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-3709969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-3711122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-3770304,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-6304082,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-6493234,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8216205-911786
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
295 ( Pt 1)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
115-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8216205-Animals,
pubmed-meshheading:8216205-Cartilage,
pubmed-meshheading:8216205-Cells, Cultured,
pubmed-meshheading:8216205-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:8216205-Collagen,
pubmed-meshheading:8216205-DNA Mutational Analysis,
pubmed-meshheading:8216205-Genes, Reporter,
pubmed-meshheading:8216205-Quail,
pubmed-meshheading:8216205-RNA, Messenger,
pubmed-meshheading:8216205-Recombinant Fusion Proteins,
pubmed-meshheading:8216205-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:8216205-Sequence Deletion,
pubmed-meshheading:8216205-Transfection,
pubmed-meshheading:8216205-Tretinoin,
pubmed-meshheading:8216205-Up-Regulation
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pubmed:year |
1993
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pubmed:articleTitle |
Alpha 2(I) collagen gene expression is up-regulated in quail chondrocytes pretreated with retinoic acid.
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pubmed:affiliation |
Dipartimento di Biochimica e Biotecnologie Mediche, University of Naples, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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