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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1993-11-22
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pubmed:abstractText |
The role of vaccinia virus nucleoside triphosphate phosphohydrolase I (NPH-I) in determining the resistance of this virus to alpha-interferon (IFN) was analyzed by using a well-defined temperature-sensitive mutant (ts36) in the NPH-I gene. Detailed analysis of viral proteins and mRNAs produced in cultured cells treated with or without IFN-alpha showed a strong inhibition by IFN of the synthesis of late virus polypeptides at the nonpermissive temperature, e.g., 83.8% inhibition for the luciferase reporter gene, which is associated with a decrease in steady-state mRNA levels. Sensitivity of ts36 to IFN was only the consequence of the point mutation in the NPH-1 gene, as shown by characterization of the rescued virus, R36. Our findings demonstrate that functional NPH-1 is required, at least in part, for resistance of vaccinia virus to IFN.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0042-6822
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
197
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pubmed:geneSymbol |
NPH-1,
NPH-I
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
485-91
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8212591-Acid Anhydride Hydrolases,
pubmed-meshheading:8212591-Adenosine Triphosphatases,
pubmed-meshheading:8212591-Animals,
pubmed-meshheading:8212591-Cell Line,
pubmed-meshheading:8212591-DNA Helicases,
pubmed-meshheading:8212591-Drug Resistance, Microbial,
pubmed-meshheading:8212591-Gene Expression,
pubmed-meshheading:8212591-Genes, Viral,
pubmed-meshheading:8212591-Haplorhini,
pubmed-meshheading:8212591-Humans,
pubmed-meshheading:8212591-Interferon-alpha,
pubmed-meshheading:8212591-L Cells (Cell Line),
pubmed-meshheading:8212591-Luciferases,
pubmed-meshheading:8212591-Mice,
pubmed-meshheading:8212591-Nucleoside-Triphosphatase,
pubmed-meshheading:8212591-RNA, Messenger,
pubmed-meshheading:8212591-RNA Probes,
pubmed-meshheading:8212591-Recombinant Proteins,
pubmed-meshheading:8212591-Transfection,
pubmed-meshheading:8212591-Vaccinia virus
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pubmed:year |
1993
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pubmed:articleTitle |
A mutation of the nucleoside triphosphate phosphohydrolase I (NPH-I) gene confers sensitivity of vaccinia virus to interferon.
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pubmed:affiliation |
Department of Biochemistry, State University of New York, Brooklyn 11203.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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