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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1993-11-9
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pubmed:abstractText |
The ability to obtain primary long-term cultures of human foetal hepatocytes maintaining liver differentiation characteristics in serum-free medium prompted us to test their susceptibility to hepatitis C virus infection. Using PCR, we detected the presence of the HCV RNA-positive strand in the supernatants and in the cells of the virus-infected hepatocyte cultures, at various times post-infection. Evidence of effective virus genome replication and multiplication was also based on the time-dependent appearance of the putative HCV RNA-negative strand, the detection of virus replicative intermediates and an increase in HCV genomic templates in the HCV-infected cells.
|
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0923-2516
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
144
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
275-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8210708-Cells, Cultured,
pubmed-meshheading:8210708-Fetus,
pubmed-meshheading:8210708-Genome, Viral,
pubmed-meshheading:8210708-Hepacivirus,
pubmed-meshheading:8210708-Humans,
pubmed-meshheading:8210708-Liver,
pubmed-meshheading:8210708-Polymerase Chain Reaction,
pubmed-meshheading:8210708-RNA, Viral,
pubmed-meshheading:8210708-Virus Cultivation,
pubmed-meshheading:8210708-Virus Replication
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pubmed:articleTitle |
Replication and multiplication of hepatitis C virus genome in human foetal liver cells.
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pubmed:affiliation |
Institute of Experimental Medicine, CNR, Roma, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|