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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0020792,
umls-concept:C0042214,
umls-concept:C0185117,
umls-concept:C0319245,
umls-concept:C0319311,
umls-concept:C0599537,
umls-concept:C0679058,
umls-concept:C1547699,
umls-concept:C1824884,
umls-concept:C2608085,
umls-concept:C2700640,
umls-concept:C2911684
|
| pubmed:dateCreated |
1994-7-13
|
| pubmed:databankReference | |
| pubmed:abstractText |
The genome of feline herpesvirus type 1 (FHV-1), the major cause of viral upper respiratory disease in cats, contains several genes encoding homologues of herpes simplex virus type 1 (HSV-1) glycoproteins. Restriction mapping studies have indicated that the group D genome of FHV-1 contains a unique short region that is 9.0 kb long. The nucleotide sequence of a 6.2 kb portion of this region was determined. Analyses of this sequence have identified five open reading frames capable of encoding homologues to HSV-1 protein kinase and glycoproteins gG, gD, gI and gE. Since gD of FHV-1 is most likely an immunologically important polypeptide, vaccinia and raccoon poxvirus recombinants expressing this glycoprotein were generated. In an indirect fluorescent antibody test these recombinants reacted strongly with a rabbit anti-FHV-1 serum. High titres of virus-neutralizing antibodies were also generated in rabbits inoculated with the vaccinia virus recombinant. A 53K viral polypeptide (gD) was detected with this antiserum on Western blots containing polypeptides from potassium tartrate-purified virions.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0022-1317
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
75 ( Pt 6)
|
| pubmed:geneSymbol |
gD,
gE,
gG,
gI
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1235-44
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8207390-Amino Acid Sequence,
pubmed-meshheading:8207390-Animals,
pubmed-meshheading:8207390-Base Sequence,
pubmed-meshheading:8207390-Cats,
pubmed-meshheading:8207390-DNA Primers,
pubmed-meshheading:8207390-Genes, Viral,
pubmed-meshheading:8207390-Genetic Vectors,
pubmed-meshheading:8207390-Herpesviridae,
pubmed-meshheading:8207390-Molecular Sequence Data,
pubmed-meshheading:8207390-Open Reading Frames,
pubmed-meshheading:8207390-Poxviridae,
pubmed-meshheading:8207390-Raccoons,
pubmed-meshheading:8207390-Recombinant Proteins,
pubmed-meshheading:8207390-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:8207390-Restriction Mapping,
pubmed-meshheading:8207390-Sequence Alignment,
pubmed-meshheading:8207390-Sequence Homology, Amino Acid,
pubmed-meshheading:8207390-Vaccinia virus,
pubmed-meshheading:8207390-Viral Envelope Proteins,
pubmed-meshheading:8207390-Viral Structural Proteins
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Identification of the feline herpesvirus type 1 (FHV-1) genes encoding glycoproteins G, D, I and E: expression of FHV-1 glycoprotein D in vaccinia and raccoon poxviruses.
|
| pubmed:affiliation |
Department of Microbiology, Michigan State University, East Lansing 48824.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|