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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1994-7-12
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pubmed:abstractText |
The phenotypic effects of selectively altering the levels of alpha B-crystallin in cultured glial cells were analyzed using sense and antisense approaches. Rat C6 glioma cells and human U-373MG glioma cells were transfected with a rat alpha B-crystallin sense cDNA or an antisense cDNA regulated by a Rous sarcoma virus promoter to alter cellular levels of alpha B-crystallin. The antisense strategy resulted in decreased alpha B-crystallin levels, as revealed by Western blot and immunocytochemical analyses. The reduced alpha B-crystallin expression was accompanied by alterations in cellular phenotype: (a) a reduction of cell size and/or a slender cell morphology; (b) a disorganized microfilament network; and (c) a reduction of cell adhesiveness. Like HSP27, the presence of additional alpha B-crystallin protein confers a thermoresistant phenotype to stable transfectants. Thus, alpha B-crystallin in glioma cells plays a role in their thermal resistance and may contribute to the stability of cytoskeletal organization.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8207065-1381680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8207065-1424733,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8207065-98070
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9525
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
125
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1385-93
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8207065-Animals,
pubmed-meshheading:8207065-Cell Adhesion,
pubmed-meshheading:8207065-Cell Line,
pubmed-meshheading:8207065-Crystallins,
pubmed-meshheading:8207065-Cytoskeleton,
pubmed-meshheading:8207065-DNA, Antisense,
pubmed-meshheading:8207065-DNA, Complementary,
pubmed-meshheading:8207065-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:8207065-Heat-Shock Proteins,
pubmed-meshheading:8207065-Hot Temperature,
pubmed-meshheading:8207065-Humans,
pubmed-meshheading:8207065-Neuroglia,
pubmed-meshheading:8207065-Rats,
pubmed-meshheading:8207065-Transfection,
pubmed-meshheading:8207065-Transformation, Genetic,
pubmed-meshheading:8207065-Tumor Cells, Cultured
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pubmed:year |
1994
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pubmed:articleTitle |
Sense and antisense modification of glial alpha B-crystallin production results in alterations of stress fiber formation and thermoresistance.
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