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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
24
|
| pubmed:dateCreated |
1994-7-14
|
| pubmed:databankReference | |
| pubmed:abstractText |
The sialyl Lewis x determinant (NeuAc alpha 2,3Gal beta 1, 4[Fuc alpha 1,3]GlcNAc) is an essential component of leukocyte counterreceptors for E-selectin and P-selectin. The final step in sialyl Lewis x synthesis is catalyzed by alpha-1,3-fucosyltransferases acting on sialylated glycoconjugate precursors. Cultured human leukocytic cell lines express an alpha-1,3-fucosyltransferase gene termed Fuc-TIV or ELFT but do not express the other three cloned human alpha-1,3-fucosyltransferase genes to any significant degree. The physiological role of Fuc-TIV/ELFT in sialyl Lewis x biosynthesis is uncertain, however, since it can catalyze the synthesis of this determinant in some, but not all, transfected cell lines in a manner that is dependent upon the glycosylation phenotype of the host cell. We report here the molecular cloning of a cDNA encoding a new human leukocyte alpha-1,3-fucosyltransferase, termed Fuc-TVII, capable of synthesizing the sialyl Lewis x moiety. The cDNA sequence predicts a 341-amino acid-long type II transmembrane protein typical of mammalian glycosyltransferases. When expressed in mammalian cells, the Fuc-TVII cDNA directs the synthesis of cell surface sialyl Lewis x moieties but not the Lewis x, Lewis a, sialyl Lewis a, or VIM-2 determinants. Fuc-TVII can efficiently utilize alpha-2,3-sialyllactosamine in vitro to form the sialyl Lewis x tetrasaccharide but does not utilize lactosamine to form the Lewis x moiety. Northern blot analyses show that the Fuc-TVII gene is transcribed in HL-60 cells, a human promyelocytic cell line, and in YT cells, a natural killer-like cell line. Fuc-TVII represents a leukocytic alpha-1,3-fucosyltransferase that can participate in selectin ligand synthesis via its ability to catalyze the synthesis of sialyl Lewis x determinants.
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| pubmed:grant | |
| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5-acetylneuraminyl-(2-3)-galactosyl-...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Fucosyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Oligosaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/galactoside 3-fucosyltransferase
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
17
|
| pubmed:volume |
269
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
16789-94
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8207002-Amino Acid Sequence,
pubmed-meshheading:8207002-Animals,
pubmed-meshheading:8207002-Base Sequence,
pubmed-meshheading:8207002-CHO Cells,
pubmed-meshheading:8207002-Carbohydrate Sequence,
pubmed-meshheading:8207002-Cell Line,
pubmed-meshheading:8207002-Cercopithecus aethiops,
pubmed-meshheading:8207002-Chromosome Mapping,
pubmed-meshheading:8207002-Chromosomes, Human, Pair 11,
pubmed-meshheading:8207002-Cloning, Molecular,
pubmed-meshheading:8207002-Cricetinae,
pubmed-meshheading:8207002-DNA,
pubmed-meshheading:8207002-DNA, Complementary,
pubmed-meshheading:8207002-DNA Primers,
pubmed-meshheading:8207002-Fucosyltransferases,
pubmed-meshheading:8207002-Humans,
pubmed-meshheading:8207002-Killer Cells, Natural,
pubmed-meshheading:8207002-Leukemia, Promyelocytic, Acute,
pubmed-meshheading:8207002-Leukocytes,
pubmed-meshheading:8207002-Mice,
pubmed-meshheading:8207002-Molecular Sequence Data,
pubmed-meshheading:8207002-Oligosaccharides,
pubmed-meshheading:8207002-Polymerase Chain Reaction,
pubmed-meshheading:8207002-Protein Structure, Secondary,
pubmed-meshheading:8207002-Recombinant Proteins,
pubmed-meshheading:8207002-Sequence Homology, Amino Acid,
pubmed-meshheading:8207002-Substrate Specificity,
pubmed-meshheading:8207002-Transfection,
pubmed-meshheading:8207002-Tumor Cells, Cultured
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Molecular cloning of a cDNA encoding a novel human leukocyte alpha-1,3-fucosyltransferase capable of synthesizing the sialyl Lewis x determinant.
|
| pubmed:affiliation |
Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor 48109.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|