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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1994-7-11
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pubmed:abstractText |
The mechanism whereby small resting (high buoyant density) murine B cells are induced to express interleukin-2 receptors (IL-2R) and to respond to IL-2 was addressed by staining with anti-IL-2R alpha and -IL-2R beta monoclonal antibodies (mAb), and using receptor-specific cDNA probes. Resting B cells expressed undetectable levels of both IL-2R alpha and beta chains on their surface and did not respond to IL-2, even at supra-physiological concentrations. Sepharose-coupled, but not streptavidin-cross-linked, plastic-adsorbed or soluble, anti-mu up-regulated the expression of IL-2R alpha and beta chains and mRNA to levels comparable to those seen in activated T cells. Anti-mu-stimulated B cells responded to IL-2 by incorporation of [3H]thymidine and high rate immunoglobulin (Ig) secretion. Both IL-5 (at optimal concentration) and suboptimal lipopolysaccharide (LPS; 20 ng/ml) induced surface expression of IL-2R alpha. The level of expression induced by IL-5 was equivalent to that on anti-Ig-activated B cells. Neither stimulus induced detectable expression of IL-2R beta, and neither induced B cells to respond to IL-2. IL-2R alpha expression was strongly enhanced, and low levels of IL-2R beta staining and mRNA were induced by the combination of LPS plus IL-5. LPS+IL-5-treated B cells responded to IL-2 by Ig secretion. This indicates that B cells regulate their responsiveness to IL-2 similarly to T cells, via the combined level of expression of IL-2R beta and IL-2R alpha. The synergy between IL-5 and LPS for B-cell responses shows a requirement for complementary stimuli such as would be provided by cytokines, and either cellular interaction or antigen recognition in regulation of B-cell responsiveness to IL-2.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-1373502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-1588041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-1597317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-1597318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-1826636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-1918958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-2129904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-2145852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-2155425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-2419430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-2467936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-2580901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-2786996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-2787531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-2831066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-2950524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-2952720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-2965645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-2965646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-2981272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-3110787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-3128631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-3263422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-3284815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-3919312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-3921620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-393521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-398327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-6256358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-6412230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-6434689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-6447723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-6605533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-6807696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8206511-6979046
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
81
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
373-80
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8206511-Animals,
pubmed-meshheading:8206511-B-Lymphocytes,
pubmed-meshheading:8206511-Cells, Cultured,
pubmed-meshheading:8206511-Drug Synergism,
pubmed-meshheading:8206511-Female,
pubmed-meshheading:8206511-Interleukin-2,
pubmed-meshheading:8206511-Interleukin-5,
pubmed-meshheading:8206511-Lipopolysaccharides,
pubmed-meshheading:8206511-Lymphocyte Activation,
pubmed-meshheading:8206511-Mice,
pubmed-meshheading:8206511-Mice, Inbred C57BL,
pubmed-meshheading:8206511-RNA, Messenger,
pubmed-meshheading:8206511-Receptors, Antigen, B-Cell,
pubmed-meshheading:8206511-Receptors, Interleukin-2,
pubmed-meshheading:8206511-Up-Regulation
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pubmed:year |
1994
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pubmed:articleTitle |
The acquisition of cytokine responsiveness by murine B cells: a role for antigen and IL-5 in the induction of IL-2 receptors.
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pubmed:affiliation |
Department of Medicine, McGill University, Montreal, Quebec, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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