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rdf:type |
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lifeskim:mentions |
umls-concept:C0001455,
umls-concept:C0007634,
umls-concept:C0010453,
umls-concept:C0017262,
umls-concept:C0035696,
umls-concept:C0039005,
umls-concept:C0039654,
umls-concept:C0040132,
umls-concept:C0041904,
umls-concept:C0162493,
umls-concept:C0185117,
umls-concept:C0596637,
umls-concept:C1623117,
umls-concept:C2003941,
umls-concept:C2911684
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pubmed:issue |
2
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pubmed:dateCreated |
1994-7-11
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pubmed:abstractText |
In this study, we examined whether the protein kinase C (PKC) pathway could interfere with the regulation of Gs protein in porcine thyroid cells. The two days culture of cells with 12-O-tetradecanoylphorbol 13-acetate (TPA) (0.1 microM) alone neither affected adenylyl cyclase activity, nor the level of Gs alpha protein in membranes when compared with control cells. The co-addition of TPA with thyrotropin (TSH) (1 mU/ml) or forskolin (fk) (10 microM) in the culture medium, abolished the stimulatory effects of either agonists on the activation of adenylyl cyclase by fk or [AlF4]- and on the increase of Gs alpha protein. By contrast, TPA had effects neither on the Gi-dependent inhibition of adenylyl cyclase nor on Gi alpha proteins levels. The level of Gs alpha mRNA measured by Northern blot analysis was increased (200%) in TSH- or fk-treated cells and this increase was counteracted by TPA. The effects of TPA observed after 6-9 h of contact with cells were mimicked by mezerezin, a non-phorbol protein kinase C activator and blocked by bisindolylmaleimide a specific protein kinase C inhibitor (GF 109203X). These results suggest that the activation of the PKC pathway prevents the cAMP-dependent up-regulation of Gs alpha protein and Gs alpha mRNA expression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Diterpenes,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Terpenes,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/Thyrotropin,
http://linkedlifedata.com/resource/pubmed/chemical/mezerein
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0303-7207
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
99
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
229-35
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8206331-Adenylate Cyclase,
pubmed-meshheading:8206331-Animals,
pubmed-meshheading:8206331-Blotting, Northern,
pubmed-meshheading:8206331-Cells, Cultured,
pubmed-meshheading:8206331-Cyclic AMP,
pubmed-meshheading:8206331-Diterpenes,
pubmed-meshheading:8206331-Enzyme Activation,
pubmed-meshheading:8206331-Forskolin,
pubmed-meshheading:8206331-GTP-Binding Proteins,
pubmed-meshheading:8206331-Gene Expression Regulation,
pubmed-meshheading:8206331-Kinetics,
pubmed-meshheading:8206331-Protein Kinase C,
pubmed-meshheading:8206331-RNA, Messenger,
pubmed-meshheading:8206331-Swine,
pubmed-meshheading:8206331-Terpenes,
pubmed-meshheading:8206331-Tetradecanoylphorbol Acetate,
pubmed-meshheading:8206331-Thyroid Gland,
pubmed-meshheading:8206331-Thyrotropin
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pubmed:year |
1994
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pubmed:articleTitle |
12-O-tetradecanoyl-phorbol-13-acetate (TPA) counteracts the cAMP up-regulation of the expression of the stimulatory guanine nucleotide binding protein (Gs alpha) and Gs alpha messenger RNA in cultured pig thyroid cells.
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pubmed:affiliation |
Unité de Recherche sur la glande thyröide et la régulation hormonale, U96 INSERM, Le Kremlin-Bicêtre, France.
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pubmed:publicationType |
Journal Article
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