Linked Life Data

8205234

Source:http://linkedlifedata.com/resource/pubmed/id/8205234

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1994-7-12
pubmed:abstractText
A sensitive, selective and validated method for the enantioselective determination of (+)- and (-)-nisoldipine in rat, mouse and dog plasma following administration of nisoldipine racemate is described. The alkalized plasma samples containing [13C4]nisoldipine racemate as internal standard (ISTD) were extracted once with toluene. The enantiomers of nisoldipine were quantitatively separated by high-performance liquid chromatography on a 250 x 2 mm I.D. column containing tris(4-methylbenzoate)-modified cellulose on silica. The fractions containing either the (+) or (-)-enantiomer of the analyte and [13C4]ISTD were analysed by gas chromatography with mass-selective detection in the single-ion monitoring mode. The limits of determination and detection were 0.5 and 0.2 ng/ml, respectively, the total precision was better than 7% (R.S.D. at 5 and 50 ng/ml, n = 35) and the accuracy was better than 10% (0.5-100 ng/ml, n = 23). The sum of the concentrations of the enantiomers determined with this assay corresponds to the concentration of the racemate determined independently by capillary gas chromatography with electron-capture detection (accuracy better than 15%, 1-80 ng/ml). The method was used for the analysis of more than 500 plasma samples obtained from toxicokinetic studies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
666
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
241-8
pubmed:dateRevised
2009-1-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Enantioselective assay for the determination of nisoldipine in dog, rat and mouse plasma by chiral microbore high-performance liquid chromatography combined with gas chromatography-mass spectrometry.
pubmed:affiliation
Institute of Pharmacokinetics, Pharma Research Centre, Bayer AG, Wuppertal, Germany.
pubmed:publicationType
Journal Article