8203495

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5 Pt 1

Na(+)-K(+)-activated adenosine triphosphatase (Na(+)-K(+)-ATPase) is the integral membrane protein that maintains the Na(+)-K(+) electrochemical gradient across the plasma membrane. Because of the importance of the Na(+)-K(+) electrochemical gradient to fundamental and specialized cell functions, we investigated the cell-specific modulation of Na(+)-K(+)-ATPase alpha-subunit isoform (alpha 1, alpha 2, and alpha 3) gene expression in different stages of postimplantation mouse embryos and neonatal rat tissues by in situ hybridization with use of isoform-specific rat-derived antisense RNA probes. At early organogenesis (9.5-10.5 days postcoitus), we demonstrated generalized coexpression of alpha 1- and alpha 2-isoforms throughout the mouse embryo with greater levels in the developing but already functional heart, in contrast to the distinct spatially restricted alpha 3-isoform gene expression in the early developing neural tube. At midorganogenesis (15.5-16.5 days postcoitus), differential spatial variation in alpha 1-, alpha 2-, and alpha 3-isoform gene expression was already evident in all organs. Interestingly, region-specific expression patterns within single cell types were noted throughout development and were exemplified by 1) alpha 3-isoform gene expression in marginal cells of the 10.5-day-postcoitus developing neural tube; 2) alpha 1-, alpha 2-, and alpha 3-isoform gene expression in cerebellar granular cells of the 4-day-old rat brain; and 3) alpha 1- and alpha 3-isoform gene expression in 4-day-old rat ventricular cardiomyocytes. These isoform-specific changes in cellular and regional Na(+)-K(+)-ATPase alpha-isoform gene expression may play an active role in development and specialized cell functions.

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0002-9513

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C1301-12

pubmed-meshheading:8203495-Animals, pubmed-meshheading:8203495-Animals, Newborn, pubmed-meshheading:8203495-Antisense Elements (Genetics), pubmed-meshheading:8203495-Blastocyst, pubmed-meshheading:8203495-Brain, pubmed-meshheading:8203495-Embryo, Mammalian, pubmed-meshheading:8203495-Embryonic and Fetal Development, pubmed-meshheading:8203495-Female, pubmed-meshheading:8203495-Gene Expression Regulation, Enzymologic, pubmed-meshheading:8203495-In Situ Hybridization, pubmed-meshheading:8203495-Isoenzymes, pubmed-meshheading:8203495-Macromolecular Substances, pubmed-meshheading:8203495-Mice, pubmed-meshheading:8203495-Organ Specificity, pubmed-meshheading:8203495-Pregnancy, pubmed-meshheading:8203495-RNA Probes, pubmed-meshheading:8203495-Rats, pubmed-meshheading:8203495-Sodium-Potassium-Exchanging ATPase

Developmental cell-specific regulation of Na(+)-K(+)-ATPase alpha 1-, alpha 2-, and alpha 3-isoform gene expression.

Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't