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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6481
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pubmed:dateCreated |
1994-7-7
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pubmed:databankReference |
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pubmed:abstractText |
The major regulator of circulating platelet levels is believed to be a cytokine termed thrombopoietin. It is thought to be a lineage-specific cytokine affecting the proliferation and maturation of committed cells resulting in the production of megakaryocytes and platelets. Despite considerable efforts by a number of laboratories, the unequivocal identification of thrombopoietin has proven elusive. Here we report the functional cloning of a murine complementary DNA encoding a ligand for the receptor encoded by the c-mpl proto-oncogene (c-Mpl). The encoded polypeptide has a predicted molecular mass of 35,000 (M(r) 35K). The protein has a novel two-domain structure with an amino-terminal domain homologous with erythropoietin and a carboxy-terminal domain rich in serine, threonine and proline residues and containing seven potential N-linked glycosylation sites. Intraperitoneal injections of mice with recombinant protein increase circulating platelet levels by greater than fourfold after 7 days. These results along with those presented in the accompanying report strongly suggest that the ligand for c-Mpl is thrombopoietin.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Erythropoietin,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/MPL protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Sorting Signals,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytokine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Thrombopoietin,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Thrombopoietin
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0028-0836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
369
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
565-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8202158-Amino Acid Sequence,
pubmed-meshheading:8202158-Animals,
pubmed-meshheading:8202158-Base Sequence,
pubmed-meshheading:8202158-Blood Platelets,
pubmed-meshheading:8202158-Cell Differentiation,
pubmed-meshheading:8202158-Cell Line,
pubmed-meshheading:8202158-Cloning, Molecular,
pubmed-meshheading:8202158-Cricetinae,
pubmed-meshheading:8202158-DNA, Complementary,
pubmed-meshheading:8202158-Erythropoietin,
pubmed-meshheading:8202158-Humans,
pubmed-meshheading:8202158-Ligands,
pubmed-meshheading:8202158-Mice,
pubmed-meshheading:8202158-Mice, Inbred BALB C,
pubmed-meshheading:8202158-Molecular Sequence Data,
pubmed-meshheading:8202158-Mutation,
pubmed-meshheading:8202158-Neoplasm Proteins,
pubmed-meshheading:8202158-Protein Sorting Signals,
pubmed-meshheading:8202158-Proto-Oncogene Proteins,
pubmed-meshheading:8202158-Receptors, Cytokine,
pubmed-meshheading:8202158-Receptors, Immunologic,
pubmed-meshheading:8202158-Receptors, Thrombopoietin,
pubmed-meshheading:8202158-Recombinant Proteins,
pubmed-meshheading:8202158-Thrombopoietin
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pubmed:year |
1994
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pubmed:articleTitle |
Cloning and expression of murine thrombopoietin cDNA and stimulation of platelet production in vivo.
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pubmed:affiliation |
ZymoGenetics Corporation, Seattle, Washington 98105.
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pubmed:publicationType |
Journal Article
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