|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1994-7-7
|
|
pubmed:abstractText |
The effects of the fatty acid inhibitor 4-bromocrotonic acid (4-BCA) on glucose utilization was studied in isolated rat myocytes. In contrast to its potent inhibition of [1-14C]palmitate oxidation, 4-BCA strongly stimulated the oxidation of [1-14C]glucose and [2-14C]-pyruvate in a concentration-dependent manner. At a concentration of 300 microM, 4-BCA increased glucose oxidation threefold and that of pyruvate oxidation twofold. The rate of transport of [U-14C]-2-deoxyglucose was significantly stimulated by 4-BCA. The transport of 2-deoxyglucose was increased sevenfold with 200 microM 4-BCA, whereas insulin (10 microU)/ml enhanced 2-deoxyglucose transport twofold. The addition of insulin to myocytes preincubated with 4-BCA did not further increase glucose transport. Cytochalasin B and anti-GLUT 4 antibody decreased the 4-BCA-induced stimulation of glucose transport. These results suggest that the stimulation of 2-deoxy-glucose transport by 4-BCA occurs through an increase in the activity of insulin-responsive glucose transporters, GLUT 4, in the sarcolemmal membrane.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-bromocrotonic acid,
http://linkedlifedata.com/resource/pubmed/chemical/Crotonic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyglucose,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose Transporter Type 4,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharide Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Palmitic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Palmitic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Pyruvates,
http://linkedlifedata.com/resource/pubmed/chemical/Slc2a4 protein, rat
|
|
pubmed:status |
MEDLINE
|
|
pubmed:issn |
0265-5985
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
22
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
11-9
|
|
pubmed:dateRevised |
2011-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:8200180-Animals,
pubmed-meshheading:8200180-Biological Transport,
pubmed-meshheading:8200180-Cells, Cultured,
pubmed-meshheading:8200180-Crotonic Acids,
pubmed-meshheading:8200180-Deoxyglucose,
pubmed-meshheading:8200180-Glucose,
pubmed-meshheading:8200180-Glucose Transporter Type 4,
pubmed-meshheading:8200180-Heart,
pubmed-meshheading:8200180-Insulin,
pubmed-meshheading:8200180-Kinetics,
pubmed-meshheading:8200180-Male,
pubmed-meshheading:8200180-Monosaccharide Transport Proteins,
pubmed-meshheading:8200180-Muscle Proteins,
pubmed-meshheading:8200180-Myocardium,
pubmed-meshheading:8200180-Oxidation-Reduction,
pubmed-meshheading:8200180-Palmitic Acid,
pubmed-meshheading:8200180-Palmitic Acids,
pubmed-meshheading:8200180-Pyruvates,
pubmed-meshheading:8200180-Rats,
pubmed-meshheading:8200180-Rats, Sprague-Dawley,
pubmed-meshheading:8200180-Sarcolemma
|
|
pubmed:year |
1993
|
|
pubmed:articleTitle |
Stimulation of myocyte insulin-responsive glucose transporters by the inhibition of fatty acid oxidation.
|
|
pubmed:affiliation |
Department of Surgery, Duke University Medical Center, Durham, NC 27710.
|
|
pubmed:publicationType |
Journal Article
|
