Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
1994-6-28
|
| pubmed:abstractText |
The genome of the non-cardiovirulent coxsackievirus B3 (CVB3) strain CVB3/0 was cloned and sequenced to aid in the elucidation of the viral genetic basis for the CVB3 cardiovirulent phenotype. Reverse-transcribed sub-genomic complementary DNA (cDNA) fragments were enzymatically amplified using generic oligonucleotide primers and were assembled as a complete infectious genomic copy (pCVB3-0) downstream of the T7 RNA polymerase promoter. Positive-strand viral RNA transcribed from pCVB3-0 using T7 RNA polymerase and transfected into HeLa cells produced infectious virus (CVB3/0c). No differences in phenotype were observed comparing growth of CVB3/0c to the parental CVB3/0 in HeLa single-step growth curves, virus yields, or plaque size. When inoculated into C3H/HeJ mice, CVB3/0c achieved cardiac titers equivalent to the parental CVB3/0 and like the parental virus, demonstrated a non-cardiovirulent phenotype. The nucleotide sequence of the cloned CVB3/0 genome was determined and compared to the genomes of infectious cDNA clones of cardiovirulent CVB3 strains. Two consistent differences among nucleotides in non-translated regions and 8 amino acid differences relative to two well-characterized infectious cDNA copies of genomes from cardiovirulent CVB3 strains were identified.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0304-8608
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
135
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
115-30
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8198437-Animals,
pubmed-meshheading:8198437-Base Sequence,
pubmed-meshheading:8198437-Capsid,
pubmed-meshheading:8198437-Cloning, Molecular,
pubmed-meshheading:8198437-DNA, Complementary,
pubmed-meshheading:8198437-DNA, Viral,
pubmed-meshheading:8198437-DNA Primers,
pubmed-meshheading:8198437-Enterovirus B, Human,
pubmed-meshheading:8198437-Genome, Viral,
pubmed-meshheading:8198437-HeLa Cells,
pubmed-meshheading:8198437-Heart,
pubmed-meshheading:8198437-Humans,
pubmed-meshheading:8198437-Male,
pubmed-meshheading:8198437-Mice,
pubmed-meshheading:8198437-Mice, Inbred C3H,
pubmed-meshheading:8198437-Molecular Sequence Data,
pubmed-meshheading:8198437-Restriction Mapping,
pubmed-meshheading:8198437-Species Specificity,
pubmed-meshheading:8198437-Transfection,
pubmed-meshheading:8198437-Viral Nonstructural Proteins,
pubmed-meshheading:8198437-Virus Replication
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
An infectious cDNA copy of the genome of a non-cardiovirulent coxsackievirus B3 strain: its complete sequence analysis and comparison to the genomes of cardiovirulent coxsackieviruses.
|
| pubmed:affiliation |
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|