| pubmed-article:8195701 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8195701 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:8195701 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:8195701 | lifeskim:mentions | umls-concept:C0026473 | lld:lifeskim |
| pubmed-article:8195701 | lifeskim:mentions | umls-concept:C0079633 | lld:lifeskim |
| pubmed-article:8195701 | lifeskim:mentions | umls-concept:C1704259 | lld:lifeskim |
| pubmed-article:8195701 | lifeskim:mentions | umls-concept:C1157338 | lld:lifeskim |
| pubmed-article:8195701 | lifeskim:mentions | umls-concept:C0677647 | lld:lifeskim |
| pubmed-article:8195701 | lifeskim:mentions | umls-concept:C1705987 | lld:lifeskim |
| pubmed-article:8195701 | lifeskim:mentions | umls-concept:C0079411 | lld:lifeskim |
| pubmed-article:8195701 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:8195701 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:8195701 | pubmed:dateCreated | 1994-6-24 | lld:pubmed |
| pubmed-article:8195701 | pubmed:abstractText | The highly regulated enzyme HMG-CoA reductase generates mevalonate, the precursor of a complex series of isoprenoids that posttranslationally modify (isoprenylate) certain proteins (e.g., the low-molecular-weight GTP-binding proteins) or that are incorporated into cholesterol and other end products. We recently reported that isoprenoids are required for NADPH oxidase activity in granulocytes via LMW GTP-binding protein isoprenylation. In this study, we evaluated the effects of isoprenoid depletion on the expression of proinflammatory genes in human monocytic THP-1 cells. We selected conditions under which pretreatment for 24 h with isoprenoid synthesis inhibitors (HMG-CoA reductase inhibitor lovastatin or compactin at 10 microM) did not compromise cell viability but markedly suppressed H2O2 generation. Under these conditions interleukin-8 (IL-8) production was attenuated (by 50-90%) in response to lipopolysaccharide, granulocyte-macrophage colony-stimulating factor, and phorbol myristate acetate. Coincubation of reductase inhibitor-treated cells with mevalonate prevented the attenuation of IL-8 production by reductase inhibitors. The effects of isoprenoid depletion on cytokine production were selective: IL-1 beta generation was not inhibited but the production of IL-6 and IL-8 was concomitantly suppressed. IL-8 induction was suppressed at least in part through attenuation of the increase in mRNA in stimulated cells. We conclude that isoprenoid generation through the mevalonate pathway is a requirement for IL-8 induction by activated monocytic cells in vitro. Isoprenylation inhibitors have the potential to alter monocyte proinflammatory function. | lld:pubmed |
| pubmed-article:8195701 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8195701 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8195701 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8195701 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8195701 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8195701 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8195701 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8195701 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8195701 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8195701 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8195701 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8195701 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8195701 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8195701 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8195701 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:8195701 | pubmed:issn | 0741-5400 | lld:pubmed |
| pubmed-article:8195701 | pubmed:author | pubmed-author:BokochG MGM | lld:pubmed |
| pubmed-article:8195701 | pubmed:author | pubmed-author:SantoroDD | lld:pubmed |
| pubmed-article:8195701 | pubmed:author | pubmed-author:BarryMMJr | lld:pubmed |
| pubmed-article:8195701 | pubmed:author | pubmed-author:TerkeltaubRR | lld:pubmed |
| pubmed-article:8195701 | pubmed:author | pubmed-author:SolanJJ | lld:pubmed |
| pubmed-article:8195701 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8195701 | pubmed:volume | 55 | lld:pubmed |
| pubmed-article:8195701 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8195701 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8195701 | pubmed:pagination | 749-55 | lld:pubmed |
| pubmed-article:8195701 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:8195701 | pubmed:meshHeading | pubmed-meshheading:8195701-... | lld:pubmed |
| pubmed-article:8195701 | pubmed:meshHeading | pubmed-meshheading:8195701-... | lld:pubmed |
| pubmed-article:8195701 | pubmed:meshHeading | pubmed-meshheading:8195701-... | lld:pubmed |
| pubmed-article:8195701 | pubmed:meshHeading | pubmed-meshheading:8195701-... | lld:pubmed |
| pubmed-article:8195701 | pubmed:meshHeading | pubmed-meshheading:8195701-... | lld:pubmed |
| pubmed-article:8195701 | pubmed:meshHeading | pubmed-meshheading:8195701-... | lld:pubmed |
| pubmed-article:8195701 | pubmed:meshHeading | pubmed-meshheading:8195701-... | lld:pubmed |
| pubmed-article:8195701 | pubmed:meshHeading | pubmed-meshheading:8195701-... | lld:pubmed |
| pubmed-article:8195701 | pubmed:meshHeading | pubmed-meshheading:8195701-... | lld:pubmed |
| pubmed-article:8195701 | pubmed:meshHeading | pubmed-meshheading:8195701-... | lld:pubmed |
| pubmed-article:8195701 | pubmed:meshHeading | pubmed-meshheading:8195701-... | lld:pubmed |
| pubmed-article:8195701 | pubmed:meshHeading | pubmed-meshheading:8195701-... | lld:pubmed |
| pubmed-article:8195701 | pubmed:meshHeading | pubmed-meshheading:8195701-... | lld:pubmed |
| pubmed-article:8195701 | pubmed:meshHeading | pubmed-meshheading:8195701-... | lld:pubmed |
| pubmed-article:8195701 | pubmed:meshHeading | pubmed-meshheading:8195701-... | lld:pubmed |
| pubmed-article:8195701 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8195701 | pubmed:articleTitle | Role of the mevalonate pathway of isoprenoid synthesis in IL-8 generation by activated monocytic cells. | lld:pubmed |
| pubmed-article:8195701 | pubmed:affiliation | Veterans Affairs Medical Center, San Diego, California 92161. | lld:pubmed |
| pubmed-article:8195701 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8195701 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8195701 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:8195701 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8195701 | lld:pubmed |
