Linked Life Data

8195332

Source:http://linkedlifedata.com/resource/pubmed/id/8195332

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-6-30
pubmed:abstractText
The metabolic derangements of pregnancies complicated by diabetes mellitus, specifically hyperglycaemia and hyperketonaemia, are known to be teratogenic during the period of organogenesis in animals. We have shown previously that poorly controlled diabetes mellitus impairs in-vivo and in-vitro mouse preimplantation embryo growth, and that culturing embryos in elevated glucose concentrations only partially recreates this developmental delay. To extend this observation we examined the effect on mouse preimplantation embryo growth of elevated concentrations of other metabolic intermediates, which may be deranged in diabetes mellitus, namely lipids, lactate, glycerol, amino acids, and ketones. Two-cell embryos from ovulation-induced B6C3F1 mice were cultured for 72 h in the presence of added lipids (250 mg/dl), lactate (5 mM), glycerol (160 microM) or mixed amino acids (8.5% travasol, 7 mM) and showed no significant difference in growth over 72 h versus their control groups. However, growth of preimplantation embryos in acetoacetate (10 mM) or in the racemic mixture of DL-beta-hydroxybutyrate (16 and 32 mM) revealed marked retardation versus controls when assessed either by distribution of developmental stages over time (24, 48, 72 h, P < 0.001) or by the difference in the average rank of sums indicating a delay in maturation (P < 0.0001). We conclude that elevated ketone concentrations adversely affect preimplantation embryo development. These findings extend previous studies which correlate uncontrolled diabetes mellitus as well as hyperglycaemia with abnormal organogenesis, and demonstrate that exposure to metabolic derangements may also hinder reproductive performance at even earlier stages in gestation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0268-1161
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
113-21
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed-meshheading:8195332-3-Hydroxybutyric Acid, pubmed-meshheading:8195332-Acetoacetates, pubmed-meshheading:8195332-Amino Acids, pubmed-meshheading:8195332-Animals, pubmed-meshheading:8195332-Butyrates, pubmed-meshheading:8195332-Cleavage Stage, Ovum, pubmed-meshheading:8195332-Culture Techniques, pubmed-meshheading:8195332-Diabetes Mellitus, Experimental, pubmed-meshheading:8195332-Embryonic Development, pubmed-meshheading:8195332-Female, pubmed-meshheading:8195332-Glycerol, pubmed-meshheading:8195332-Hydroxybutyrates, pubmed-meshheading:8195332-Lactates, pubmed-meshheading:8195332-Lactic Acid, pubmed-meshheading:8195332-Lipids, pubmed-meshheading:8195332-Mice, pubmed-meshheading:8195332-Mice, Inbred Strains, pubmed-meshheading:8195332-Pregnancy, pubmed-meshheading:8195332-Pregnancy in Diabetics
pubmed:year
1994
pubmed:articleTitle
Manifestations of diabetes mellitus on mouse preimplantation development: effect of elevated concentration of metabolic intermediates.
pubmed:affiliation
Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, CT 06510.
pubmed:publicationType
Journal Article