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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1994-6-29
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pubmed:abstractText |
We have purified a serine proteinase from the membrane of U-937 cells that was inhibited in a tight-binding manner by recombinant gp120 and by peptides mimicking the V3 loop of gp120 [(1993) FEBS Lett. 317, 167-172]. This proteinase has now been characterized, both structurally and functionally. It has a dual trypsin- and chymotrypsin-like specificity, and N-terminal sequence analysis of the first 32 residues indicates complete identity with leukocyte cathepsin G. Cathepsin G-like material was located at the surface of U-937 cells using a monoclonal antibody directed against leukocyte cathepsin G, and polyclonal anti-cathepsin G antibodies precipitated the purified proteinase. However, the U-937 enzyme differs slightly from commercial leukocyte cathepsin G in its apparent M(r) because of different glycosylation. No other protein structurally related to cathepsin G was found upon screening a U-937 cDNA library using several oligonucleotide probes constructed from the membrane proteinase N-terminal amino acid sequence. The possible interaction of a cathepsin G-like proteinase at the surface of U-937 cells with the V3 loop of HIV-1 gp120 is discussed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
345
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
81-6
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8194606-Amino Acid Sequence,
pubmed-meshheading:8194606-Base Sequence,
pubmed-meshheading:8194606-Cathepsin G,
pubmed-meshheading:8194606-Cathepsins,
pubmed-meshheading:8194606-Cell Membrane,
pubmed-meshheading:8194606-Cross Reactions,
pubmed-meshheading:8194606-DNA, Complementary,
pubmed-meshheading:8194606-HIV Envelope Protein gp120,
pubmed-meshheading:8194606-Humans,
pubmed-meshheading:8194606-Immunoblotting,
pubmed-meshheading:8194606-Leukocytes,
pubmed-meshheading:8194606-Molecular Sequence Data,
pubmed-meshheading:8194606-Oligonucleotide Probes,
pubmed-meshheading:8194606-Peptide Fragments,
pubmed-meshheading:8194606-Sequence Analysis,
pubmed-meshheading:8194606-Serine Endopeptidases,
pubmed-meshheading:8194606-Substrate Specificity,
pubmed-meshheading:8194606-Tumor Cells, Cultured
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pubmed:year |
1994
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pubmed:articleTitle |
Identification of the U-937 membrane-associated proteinase interacting with the V3 loop of HIV-1 gp120 as cathepsin G.
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pubmed:affiliation |
Laboratoire d'Enzymologie, Centre National de la Recherche Scientifique URA 1334, University François Rabelais, Faculty of Medicine, Tours, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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