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rdf:type |
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lifeskim:mentions |
umls-concept:C0014533,
umls-concept:C0026237,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0035820,
umls-concept:C0205409,
umls-concept:C0337051,
umls-concept:C0600069,
umls-concept:C0851285,
umls-concept:C1151688,
umls-concept:C1509144
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pubmed:issue |
1
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pubmed:dateCreated |
1976-8-2
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pubmed:abstractText |
1. Isolated fat-cells and intact epididymal fat-pads were incubated in medium containing 45Ca2+ and the incorporation of 45Ca into mitochondrial and extramitochondrial fractions was studied. Redistribution of 45Ca between these fractions was essentially prevented by the addition of EGTA [ethanedioxybis(ethylamine)tetra-acetate] and Ruthenium Red to the sucrose-based extraction medium. 2. Incorporation of 45Ca into mitochondrial fractions of both fat-cells and fat-pads was found to be complete within 2-5 min, suggesting that mitochondria contain a pool of calcium in rapid isotopic exchange with extracellular Ca2+. This pool was about 20 times larger in mitochondria within fat-cells than within fat-pads. In fat-cells, 45Ca incorporation into the mitochondrial fraction accounted for about 34% of the total 45Ca incorporation into cells after 20 min and about 50% of the total mitochondrial calcium content measured by atomic absorption; values in fat-pads were about 7 and 20% respectively.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-1212220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-13313307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-13946593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-14087335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-171557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4118937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4243733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4306045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4312751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4321572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4343661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4353596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4358369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4366164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4375377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4375962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4377912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4393782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4407159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4417777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4429666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4452361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4462746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4632434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4658997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4659260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4734330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-4850852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-5158897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-5158898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-5776240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/819008-6018
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcimycin,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Dinitrophenols,
http://linkedlifedata.com/resource/pubmed/chemical/Egtazic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Epinephrine,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamate Dehydrogenase,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Lithium,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Pyruvate Dehydrogenase Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Ruthenium Red,
http://linkedlifedata.com/resource/pubmed/chemical/Succinates
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
154
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
209-23
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:819008-Adipose Tissue,
pubmed-meshheading:819008-Animals,
pubmed-meshheading:819008-Calcimycin,
pubmed-meshheading:819008-Calcium,
pubmed-meshheading:819008-Diabetes Mellitus, Experimental,
pubmed-meshheading:819008-Dinitrophenols,
pubmed-meshheading:819008-Egtazic Acid,
pubmed-meshheading:819008-Epididymis,
pubmed-meshheading:819008-Epinephrine,
pubmed-meshheading:819008-Extracellular Space,
pubmed-meshheading:819008-Glutamate Dehydrogenase,
pubmed-meshheading:819008-Insulin,
pubmed-meshheading:819008-Lithium,
pubmed-meshheading:819008-Male,
pubmed-meshheading:819008-Mitochondria,
pubmed-meshheading:819008-Phosphates,
pubmed-meshheading:819008-Pyruvate Dehydrogenase Complex,
pubmed-meshheading:819008-Rats,
pubmed-meshheading:819008-Ruthenium Red,
pubmed-meshheading:819008-Succinates,
pubmed-meshheading:819008-Time Factors
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pubmed:year |
1976
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pubmed:articleTitle |
Exchangeable and total calcium pools in mitochondria of rat epididymal fat-pads and isolated fat-cells. Role in the regulation of pyruvate dehydrogenase activity.
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