Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1994-6-20
pubmed:abstractText
Herpes simplex virus DNA replication proteins localize in characteristic patterns corresponding to viral DNA replication structures in the infected cell nucleus. The intranuclear spatial organization of the HSV DNA replication structures and the factors regulating their nuclear location remain to be defined. We have used the HSV ICP8 DNA-binding protein and bromodeoxyuridine labeling as markers for sites of herpesviral DNA synthesis to examine the spatial organization of these structures within the cell nucleus. Confocal microscopy and three-dimensional computer graphics reconstruction of optical series through infected cells indicated that viral DNA replication structures extend through the interior of the cell nucleus and appear to be spatially separate from the nuclear lamina. Examination of viral DNA replication structures in infected, binucleate cells showed similar or virtually identical patterns of DNA replication structures oriented along a twofold axis of symmetry between many of the sister nuclei. These results demonstrate that HSV DNA replication structures are organized in the interior of the nucleus and that their location is defined by preexisting host cell nuclear architecture, probably the internal nuclear matrix.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-14402487, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-1545814, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-1671528, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-16789121, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-168402, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-1847437, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-2173766, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-2174562, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-2408013, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-2517017, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-2535726, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-2549858, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-2557760, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-2826806, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-2847874, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-2945931, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-3020158, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-3024166, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-3027360, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-3531199, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-4550024, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-53071, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-5941279, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-6287005, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-6310045, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-6323024, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-8402887, http://linkedlifedata.com/resource/pubmed/commentcorrection/8189490-8446902
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3512-26
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Preexisting nuclear architecture defines the intranuclear location of herpesvirus DNA replication structures.
pubmed:affiliation
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.