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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1994-6-22
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pubmed:abstractText |
ErbB3 is a member of the epidermal growth factor (EGF) receptor subfamily of receptor tyrosine kinases and is believed to be a receptor for an unknown ligand. We have tested the possibility that heregulin, a growth factor possessing an EGF-like domain, is a ligand for ErbB3. We have found that the iodinated recombinant EGF-like domain of heregulin-beta 1 (125I-rHRG beta 1(177-244) bound specifically to insect cell-expressed bovine ErbB3 with a dissociation constant of 0.85 nM. Moreover, 125I-rHRG beta 1(177-244) bound to NIH3T3 fibroblasts stably transfected with bovine erbB3 with a dissociation constant of 60 pM, but did not bind to parental cells. 125I-rHRG beta 1(177-244) could be chemically cross-linked to a 170-180 kDa protein in erbB3-transfected fibroblasts, and the cross-linked product could be immunoprecipitated with antibodies specific for ErbB3. Finally, rHRG beta 1 stimulated the tyrosine phosphorylation of both ErbB3 and endogenous p185erbB2/neu in transfectants but not in parental cells. We conclude that ErbB3 is a receptor for HRG and is capable of mediating HRG-stimulated tyrosine phosphorylation of itself and p185erbB2/neu in cells that express both receptors.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Iodine Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Neuregulin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, erbB-3,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/heregulin beta1
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
269
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
14303-6
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8188716-3T3 Cells,
pubmed-meshheading:8188716-Amino Acid Sequence,
pubmed-meshheading:8188716-Animals,
pubmed-meshheading:8188716-Baculoviridae,
pubmed-meshheading:8188716-Binding Sites,
pubmed-meshheading:8188716-Carrier Proteins,
pubmed-meshheading:8188716-Cattle,
pubmed-meshheading:8188716-Cells, Cultured,
pubmed-meshheading:8188716-Cloning, Molecular,
pubmed-meshheading:8188716-Escherichia coli,
pubmed-meshheading:8188716-Glycoproteins,
pubmed-meshheading:8188716-Iodine Radioisotopes,
pubmed-meshheading:8188716-Mice,
pubmed-meshheading:8188716-Molecular Sequence Data,
pubmed-meshheading:8188716-Moths,
pubmed-meshheading:8188716-Neuregulin-1,
pubmed-meshheading:8188716-Phosphorylation,
pubmed-meshheading:8188716-Proto-Oncogene Proteins,
pubmed-meshheading:8188716-Receptor, Epidermal Growth Factor,
pubmed-meshheading:8188716-Receptor, erbB-3,
pubmed-meshheading:8188716-Transfection,
pubmed-meshheading:8188716-Tyrosine
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pubmed:year |
1994
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pubmed:articleTitle |
The erbB3 gene product is a receptor for heregulin.
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pubmed:affiliation |
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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