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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1994-6-20
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pubmed:databankReference |
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pubmed:abstractText |
The gene encoding a tripeptidase (pepT) of Lactococcus lactis subsp. cremoris (formerly subsp. lactis) MG1363 was cloned from a genomic library in pUC19 and subsequently sequenced. The tripeptidase of L. lactis was shown to be homologous to PepT of Salmonella typhimurium with 47.4% identity in the deduced amino acid sequences. L. lactis PepT was enzymatically active in Escherichia coli and allowed growth of a peptidase-negative leucine-auxotrophic E. coli strain by liberation of Leu from a tripeptide. Using a two-step integration-excision system, a pepT-negative mutant of L. lactis was constructed. No differences between the growth of the mutant and that of the wild-type strain in milk or in chemically defined medium with casein as the sole source of essential amino acids were observed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-1352755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-13742799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-1558766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-1563625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-16346027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-16346979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-16348073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-16348224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-16348281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-16348629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-16348982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-16350018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-1674655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-1674656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-1685079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-1695895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-1904438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-1905257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-2120234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-2125811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-2216800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-2410543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-2447829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-2497708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-3123462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-3162770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-3316184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-355237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-4519026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-4990652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-6304321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-6396165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-6403500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-6743336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-6773926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-7039409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-8244921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-8244951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-8320375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-8398214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-8439160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8188586-8458851
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
176
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pubmed:geneSymbol |
pepT
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2854-61
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:8188586-Peptide Hydrolases,
pubmed-meshheading:8188586-Salmonella typhimurium,
pubmed-meshheading:8188586-Escherichia coli,
pubmed-meshheading:8188586-Lactococcus lactis,
pubmed-meshheading:8188586-Base Sequence,
pubmed-meshheading:8188586-Aminopeptidases,
pubmed-meshheading:8188586-Genes, Bacterial,
pubmed-meshheading:8188586-Amino Acid Sequence,
pubmed-meshheading:8188586-Chromosomes, Bacterial,
pubmed-meshheading:8188586-Molecular Sequence Data,
pubmed-meshheading:8188586-Mutagenesis,
pubmed-meshheading:8188586-Cloning, Molecular,
pubmed-meshheading:8188586-Sequence Homology, Amino Acid,
pubmed-meshheading:8188586-Sequence Analysis, DNA,
pubmed-meshheading:8188586-Sequence Deletion
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