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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1994-6-23
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pubmed:databankReference |
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pubmed:abstractText |
The S. cerevisiae VPS10 (vacuolar protein sorting) gene encodes a type I transmembrane protein of 1577 amino acids required for the sorting of the soluble vacuolar protein carboxypeptidase Y (CPY). Mutations in VPS10 result in the selective missorting and secretion of CPY; all other vacuolar proteins tested are delivered to the vacuole in vps10 mutants. Chemical cross-linking studies demonstrate that Vps10p and the Golgi-modified precursor form of CPY directly interact. A single amino acid change in the CPY vacuolar sorting signal prevents this interaction. Vps10p also interacts with a hybrid protein containing the CPY sorting signal fused to the normally secreted enzyme invertase. Subcellular fractionation indicates that the majority of Vps10p is localized to a late Golgi compartment where vacuolar proteins are sorted. We propose that VPS10 encodes a CPY sorting receptor that executes multiple rounds of sorting by cycling between the late Golgi and a prevacuolar endosome-like compartment.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carboxypeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Cathepsin A,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PRC1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Vesicular Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/serine carboxypeptidase
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
77
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pubmed:geneSymbol |
VPS10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
579-86
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8187177-Amino Acid Sequence,
pubmed-meshheading:8187177-Carboxypeptidases,
pubmed-meshheading:8187177-Cathepsin A,
pubmed-meshheading:8187177-Cloning, Molecular,
pubmed-meshheading:8187177-Fungal Proteins,
pubmed-meshheading:8187177-Genes, Fungal,
pubmed-meshheading:8187177-Genetic Complementation Test,
pubmed-meshheading:8187177-Glycosylation,
pubmed-meshheading:8187177-Golgi Apparatus,
pubmed-meshheading:8187177-Membrane Proteins,
pubmed-meshheading:8187177-Models, Biological,
pubmed-meshheading:8187177-Molecular Sequence Data,
pubmed-meshheading:8187177-Mutation,
pubmed-meshheading:8187177-Receptors, Cell Surface,
pubmed-meshheading:8187177-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:8187177-Saccharomyces cerevisiae,
pubmed-meshheading:8187177-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:8187177-Sequence Analysis, DNA,
pubmed-meshheading:8187177-Sequence Homology, Amino Acid,
pubmed-meshheading:8187177-Spheroplasts,
pubmed-meshheading:8187177-Vacuoles,
pubmed-meshheading:8187177-Vesicular Transport Proteins
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pubmed:year |
1994
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pubmed:articleTitle |
The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene.
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pubmed:affiliation |
Division of Cellular and Molecular Medicine, University of California, San Diego School of Medicine, La Jolla 92093-0668.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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