| pubmed-article:8186466 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8186466 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:8186466 | lifeskim:mentions | umls-concept:C0442043 | lld:lifeskim |
| pubmed-article:8186466 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:8186466 | lifeskim:mentions | umls-concept:C0599220 | lld:lifeskim |
| pubmed-article:8186466 | lifeskim:mentions | umls-concept:C1947953 | lld:lifeskim |
| pubmed-article:8186466 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:8186466 | lifeskim:mentions | umls-concept:C1512032 | lld:lifeskim |
| pubmed-article:8186466 | lifeskim:mentions | umls-concept:C1428114 | lld:lifeskim |
| pubmed-article:8186466 | lifeskim:mentions | umls-concept:C2003941 | lld:lifeskim |
| pubmed-article:8186466 | lifeskim:mentions | umls-concept:C2362546 | lld:lifeskim |
| pubmed-article:8186466 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:8186466 | lifeskim:mentions | umls-concept:C2700372 | lld:lifeskim |
| pubmed-article:8186466 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:8186466 | lifeskim:mentions | umls-concept:C0086149 | lld:lifeskim |
| pubmed-article:8186466 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:8186466 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8186466 | pubmed:dateCreated | 1994-6-20 | lld:pubmed |
| pubmed-article:8186466 | pubmed:abstractText | Previous results have shown that the G alpha protein subunit G alpha 2 is required for aggregation in Dictyostelium discoideum and is essential for coupling cell-surface cAMP receptors to downstream effectors in vivo during this stage of development. G alpha 2 expresses at least four distinct transcripts that are differentially regulated during development; two of the transcripts are expressed exclusively in the multicellular stages and their expression is restricted to prestalk cells. We partially dissected the G alpha 2 promoter and identified a component that is expressed exclusively during the multicellular stages using luciferase gene fusions. When this promoter region is coupled to lacZ, beta-gal expression is restricted to the multicellular stages and localized in prestalk cells with a pattern similar to that of the ecmA prestalk-specific promoter. We show that expression in wild-type cells of the G alpha 2 mutant protein [G alpha 2(G206T)] during the early stages of development blocks aggregation and cAMP-mediated activation of adenylyl cyclase and guanylyl cyclase, suggesting it functions as a dominant negatively active G alpha subunit. When this mutant G alpha protein is expressed from the ecmA prestalk-specific promoter, abnormal stalk differentiation during culmination is observed. Expression of the mutant G alpha 2 from the SP60 prespore promoter or wild-type G alpha 2 from either the ecmA or the SP60 promoter results in no detectable phenotype. The results suggest that G alpha 2 plays an essential role during the culmination stage in prestalk cells and may mediate cAMP receptor activation of these processes during multicellular development. | lld:pubmed |
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| pubmed-article:8186466 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8186466 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8186466 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8186466 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8186466 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8186466 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8186466 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8186466 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8186466 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:8186466 | pubmed:issn | 1059-1524 | lld:pubmed |
| pubmed-article:8186466 | pubmed:author | pubmed-author:ClarkA MAM | lld:pubmed |
| pubmed-article:8186466 | pubmed:author | pubmed-author:FirtelR ARA | lld:pubmed |
| pubmed-article:8186466 | pubmed:author | pubmed-author:CarrenDD | lld:pubmed |
| pubmed-article:8186466 | pubmed:author | pubmed-author:Dharmawardhan... | lld:pubmed |
| pubmed-article:8186466 | pubmed:author | pubmed-author:Powell-Coffma... | lld:pubmed |
| pubmed-article:8186466 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8186466 | pubmed:volume | 5 | lld:pubmed |
| pubmed-article:8186466 | pubmed:geneSymbol | lacZ | lld:pubmed |
| pubmed-article:8186466 | pubmed:geneSymbol | ecmA | lld:pubmed |
| pubmed-article:8186466 | pubmed:geneSymbol | Act15 | lld:pubmed |
| pubmed-article:8186466 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8186466 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8186466 | pubmed:pagination | 7-16 | lld:pubmed |
| pubmed-article:8186466 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:8186466 | pubmed:meshHeading | pubmed-meshheading:8186466-... | lld:pubmed |
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