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rdf:type |
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lifeskim:mentions |
umls-concept:C0007589,
umls-concept:C0017262,
umls-concept:C0033684,
umls-concept:C0086149,
umls-concept:C0185117,
umls-concept:C0442043,
umls-concept:C0599220,
umls-concept:C1428114,
umls-concept:C1511938,
umls-concept:C1512032,
umls-concept:C1947953,
umls-concept:C2003941,
umls-concept:C2362546,
umls-concept:C2700372,
umls-concept:C2911684
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pubmed:issue |
1
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pubmed:dateCreated |
1994-6-20
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pubmed:abstractText |
Previous results have shown that the G alpha protein subunit G alpha 2 is required for aggregation in Dictyostelium discoideum and is essential for coupling cell-surface cAMP receptors to downstream effectors in vivo during this stage of development. G alpha 2 expresses at least four distinct transcripts that are differentially regulated during development; two of the transcripts are expressed exclusively in the multicellular stages and their expression is restricted to prestalk cells. We partially dissected the G alpha 2 promoter and identified a component that is expressed exclusively during the multicellular stages using luciferase gene fusions. When this promoter region is coupled to lacZ, beta-gal expression is restricted to the multicellular stages and localized in prestalk cells with a pattern similar to that of the ecmA prestalk-specific promoter. We show that expression in wild-type cells of the G alpha 2 mutant protein [G alpha 2(G206T)] during the early stages of development blocks aggregation and cAMP-mediated activation of adenylyl cyclase and guanylyl cyclase, suggesting it functions as a dominant negatively active G alpha subunit. When this mutant G alpha protein is expressed from the ecmA prestalk-specific promoter, abnormal stalk differentiation during culmination is observed. Expression of the mutant G alpha 2 from the SP60 prespore promoter or wild-type G alpha 2 from either the ecmA or the SP60 promoter results in no detectable phenotype. The results suggest that G alpha 2 plays an essential role during the culmination stage in prestalk cells and may mediate cAMP receptor activation of these processes during multicellular development.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1312225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1325653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1332700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1333842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1339658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1348970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1355376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1631140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1668191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1668648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1670774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1692327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1703508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1730409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1836954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1840893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1848223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1849108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1879341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1910174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1930158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1962211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1968413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1986000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1989903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-1997041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-2044155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-2110382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-2163344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-2500658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-2539262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-2539811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-2546676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-2561454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-2636139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-2672337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-2821029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-2843545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-2846388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-3025622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-3031475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-3100295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-3362676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-3405751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-3771567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-6825713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-8099335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-8275851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-8382181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-8394342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8186466-8436297
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1059-1524
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:geneSymbol |
Act15,
ecmA,
lacZ
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7-16
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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