Linked Life Data

8185917

Source:http://linkedlifedata.com/resource/pubmed/id/8185917

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1994-6-22
pubmed:abstractText
We have developed a new DNA-specific fluorochrome that is excited by visible light. This chemically uncharacterized fluorochrome readily penetrates tissue in whole mounts, gives a strong signal, is inexpensive to make and very easy to use. This preparation has several advantages over existing DNA fluorochromes in confocal laser microscopy applications. Many DNA fluorochromes are excitable by ultraviolet light. These have limited utility in confocal microscopy, where commonly used lasers produce only visible wavelengths of light. Other DNA fluorochromes are available that are excitable by visible light, but some have difficulty penetrating all cell types, while others have high background or fade quickly. This new fluorochrome overcomes these problems and has an emission spectra that makes it compatible with either rhodamine or Texas Red labeled reagents for double-label experiments. The ability to label all nuclei in a tissue and make three-dimensional reconstructions by confocal microscopy is extremely useful in examining cellular distributions within specific tissues.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0736-6205
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
434-40
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
A new visible light DNA fluorochrome for confocal microscopy.
pubmed:affiliation
University of Virginia, Charlottesville.
pubmed:publicationType
Comparative Study, Research Support, U.S. Gov't, P.H.S., Technical Report