Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1994-6-14
|
pubmed:abstractText |
We present a method that specifically records .OH formation within histones and possibly at other sites in irradiated nucleohistone. The approach uses the radiation-induced fluorescence emissions from a chromatin-conjugated .OH detector, SECCA (a succinylated derivative of coumarin), that is converted to a fluorescent derivative, 7-hydroxy-SECCA (7-OH-SECCA), after interaction with .OH in neutral aqueous solutions. It is shown that (a) the fluorescent product 7-OH-SECCA cannot be generated by direct radiation effects after gamma or neutron irradiation of SECCA; (b) when SECCA-labeled histone is complexed with DNA to form nucleohistone, the physical properties of the modified nucleohistone are similar to those of unlabeled nucleoprotein; and (c) after irradiation of SECCA-labeled nucleohistone, a linear induction of the fluorescence signal is observed within the radiation doses examined (0.3-30 Gy). Since the sample remains available for further studies after registration of the optical signal, the current approach should permit the investigator to correlate in a single sample the localization and frequency of .OH formation with the results of other assays.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Coumarins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Histones,
http://linkedlifedata.com/resource/pubmed/chemical/Hydroxyl Radical
|
pubmed:status |
MEDLINE
|
pubmed:month |
May
|
pubmed:issn |
0033-7587
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
138
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
177-85
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:8183987-Animals,
pubmed-meshheading:8183987-Cattle,
pubmed-meshheading:8183987-Coumarins,
pubmed-meshheading:8183987-DNA,
pubmed-meshheading:8183987-Fluorescent Dyes,
pubmed-meshheading:8183987-Histones,
pubmed-meshheading:8183987-Hydroxyl Radical,
pubmed-meshheading:8183987-Spectrometry, Fluorescence
|
pubmed:year |
1994
|
pubmed:articleTitle |
Quantification of radiation-induced hydroxyl radicals within nucleohistones using a molecular fluorescent probe.
|
pubmed:affiliation |
Department of Radiation Oncology, Harvard Medical School, Boston, Massachusetts 02115.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|