| pubmed-article:8183367 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8183367 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:8183367 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:8183367 | lifeskim:mentions | umls-concept:C0032521 | lld:lifeskim |
| pubmed-article:8183367 | lifeskim:mentions | umls-concept:C0230922 | lld:lifeskim |
| pubmed-article:8183367 | lifeskim:mentions | umls-concept:C1510941 | lld:lifeskim |
| pubmed-article:8183367 | lifeskim:mentions | umls-concept:C0598651 | lld:lifeskim |
| pubmed-article:8183367 | pubmed:issue | 6478 | lld:pubmed |
| pubmed-article:8183367 | pubmed:dateCreated | 1994-6-15 | lld:pubmed |
| pubmed-article:8183367 | pubmed:abstractText | Current methods for immobilizing enzymes for use in bioreactors and biosensors include adsorption on or covalent attachment to a support, micro-encapsulation, and entrapment within a membrane/film or gel. The ideal immobilization method should employ mild chemical conditions, allow for large quantities of enzyme to be immobilized, provide a large surface area for enzyme-substrate contact within a small total volume, minimize barriers to mass transport of substrate and product, and provide a chemically and mechanically robust system. Here we describe a method for enzyme immobilization that satisfies all of these criteria. We have developed a template-based synthetic method that yields hollow polymeric microcapsules of uniform diameter and length. These microcapsules are arranged in a high-density array in which the individual capsules protrude from a surface like the bristles of a brush. We have developed procedures for filling these microcapsules with high concentrations of enzymes. The enzyme-loaded microcapsule arrays function as enzymatic bioreactors in both aqueous solution and organic solvents. | lld:pubmed |
| pubmed-article:8183367 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8183367 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8183367 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8183367 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8183367 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8183367 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8183367 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8183367 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8183367 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8183367 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8183367 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8183367 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8183367 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8183367 | pubmed:month | May | lld:pubmed |
| pubmed-article:8183367 | pubmed:issn | 0028-0836 | lld:pubmed |
| pubmed-article:8183367 | pubmed:author | pubmed-author:MartinC RCR | lld:pubmed |
| pubmed-article:8183367 | pubmed:author | pubmed-author:Parthasarathy... | lld:pubmed |
| pubmed-article:8183367 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8183367 | pubmed:day | 26 | lld:pubmed |
| pubmed-article:8183367 | pubmed:volume | 369 | lld:pubmed |
| pubmed-article:8183367 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8183367 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8183367 | pubmed:pagination | 298-301 | lld:pubmed |
| pubmed-article:8183367 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8183367 | pubmed:meshHeading | pubmed-meshheading:8183367-... | lld:pubmed |
| pubmed-article:8183367 | pubmed:meshHeading | pubmed-meshheading:8183367-... | lld:pubmed |
| pubmed-article:8183367 | pubmed:meshHeading | pubmed-meshheading:8183367-... | lld:pubmed |
| pubmed-article:8183367 | pubmed:meshHeading | pubmed-meshheading:8183367-... | lld:pubmed |
| pubmed-article:8183367 | pubmed:meshHeading | pubmed-meshheading:8183367-... | lld:pubmed |
| pubmed-article:8183367 | pubmed:meshHeading | pubmed-meshheading:8183367-... | lld:pubmed |
| pubmed-article:8183367 | pubmed:meshHeading | pubmed-meshheading:8183367-... | lld:pubmed |
| pubmed-article:8183367 | pubmed:meshHeading | pubmed-meshheading:8183367-... | lld:pubmed |
| pubmed-article:8183367 | pubmed:meshHeading | pubmed-meshheading:8183367-... | lld:pubmed |
| pubmed-article:8183367 | pubmed:meshHeading | pubmed-meshheading:8183367-... | lld:pubmed |
| pubmed-article:8183367 | pubmed:meshHeading | pubmed-meshheading:8183367-... | lld:pubmed |
| pubmed-article:8183367 | pubmed:meshHeading | pubmed-meshheading:8183367-... | lld:pubmed |
| pubmed-article:8183367 | pubmed:meshHeading | pubmed-meshheading:8183367-... | lld:pubmed |
| pubmed-article:8183367 | pubmed:meshHeading | pubmed-meshheading:8183367-... | lld:pubmed |
| pubmed-article:8183367 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8183367 | pubmed:articleTitle | Synthesis of polymeric microcapsule arrays and their use for enzyme immobilization. | lld:pubmed |
| pubmed-article:8183367 | pubmed:affiliation | Department of Chemistry, Colorado State University, Fort Collins 80523. | lld:pubmed |
| pubmed-article:8183367 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8183367 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8183367 | lld:pubmed |
