Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1994-6-14
|
| pubmed:abstractText |
Taking advantage of the reverse transcriptase-polymerase chain reaction (RT-PCR), we have analyzed T cell receptor gamma-chain mRNA of synovial fluid gamma/delta T cells from patients with rheumatoid arthritis (RA) in comparison with those of peripheral blood mononuclear cells (PBMC) from RA patients and healthy individuals. The quantitative RT-PCR method in conjunction with nucleotide sequencing revealed the frequent usage of the V gamma 3 gene segment in RA synovial fluid mononuclear cells (SFMC) (p < 0.01) which in PBMC of healthy individuals occurred rarely. PBMC of most healthy individuals expressed the V gamma 9 gene predominantly (p < 0.01) as expected. However, only half of RA patients showed elevated levels of the V gamma 9 gene expression in their PBMC. The gamma-chain mRNA containing the V gamma 3 gene in RA SFMC showed no conserved junctional sequence (complementarity-determining region 3). To investigate the nature of ligands recognized by the V gamma 3-bearing T cells, we analyzed V gamma gene usage of RA SFMC, RA PBMC, and normal PBMC stimulated with Mycobacterium tuberculosis (MT) or MT plus interleukin-2 since there is mounting evidence of high reactivity of RA SFMC to MT and mycobacterial heat-shock protein 65. However, the V gamma usage appeared to be mostly V gamma 9 in RA SFMC, RA PBMC and normal PBMC. Taken together these results suggest that an as yet unknown antigen(s) (other than MT) might select gamma/delta T cells expressing the V gamma 3 gene in RA SFMC.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0014-2980
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
24
|
| pubmed:geneSymbol |
V<down>&ggr;</down>
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1122-9
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8181523-Adolescent,
pubmed-meshheading:8181523-Adult,
pubmed-meshheading:8181523-Aged,
pubmed-meshheading:8181523-Amino Acid Sequence,
pubmed-meshheading:8181523-Arthritis, Rheumatoid,
pubmed-meshheading:8181523-Base Sequence,
pubmed-meshheading:8181523-Female,
pubmed-meshheading:8181523-Gene Frequency,
pubmed-meshheading:8181523-Gout,
pubmed-meshheading:8181523-Humans,
pubmed-meshheading:8181523-Male,
pubmed-meshheading:8181523-Middle Aged,
pubmed-meshheading:8181523-Molecular Sequence Data,
pubmed-meshheading:8181523-Polymerase Chain Reaction,
pubmed-meshheading:8181523-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:8181523-Synovial Fluid,
pubmed-meshheading:8181523-T-Lymphocytes
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
The biased V gamma gene usage in the synovial fluid of patients with rheumatoid arthritis.
|
| pubmed:affiliation |
Department of Orthopedics, Hamamatsu University School of Medicine, Japan.
|
| pubmed:publicationType |
Journal Article
|