Linked Life Data

8180101

Source:http://linkedlifedata.com/resource/pubmed/id/8180101

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-6-14
pubmed:abstractText
An immunocytochemical method was developed to study in vivo induction and removal of DNA damage in a specific cell population in the epidermis of hairless mice after ultraviolet B (UVB) exposure: the immunocompetent antigen-presenting Langerhans cells. To this aim, Ia+ cells, which are representative for epidermal Langerhans cells, were compared with the bulk of epidermal cells with respect to the nuclear level of cyclobutane thymine dimers. Mouse Langerhans cells were identified with a membrane-located immunoperoxidase stain, whereas DNA and DNA damage were revealed with fluorescent nuclear stains. After a low dose of UVB (approximately 1 minimal erythema dose), dimer levels were determined both in all murine epidermal cells and in Ia+ cells separately. At 24 h after irradiation, dimer removal was still incomplete, with a persistence of approximately 50% of the initially induced dimers in epidermal cells in general, and of approximately 75% in Langerhans cells. Possible applications of the method developed and the results presented here are discussed in relation to the immunosuppressive effect of UV.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0905-4383
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8-12
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Immunochemical detection of cyclobutane thymine dimers in epidermal Langerhans cells of ultraviolet B-irradiated hairless mice.
pubmed:affiliation
TNO Medical Biological Laboratory, Rijswijk, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't