Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4 Pt 1
|
pubmed:dateCreated |
1994-6-9
|
pubmed:abstractText |
Intracellular pH (pHi) measurements in frog retinal pigment epithelium using the pH-sensitive dye 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein demonstrate that the basolateral membrane contains a pHi-sensitive Cl-HCO3 exchanger. In control Ringer solution, the removal of Cl from the basal bath alkalinized the cells by 0.07 +/- 0.03 (SD) pH units (n = 39) with an initial rate of 0.022 +/- 0.0013 pH units/min. This effect was blocked by 0.5 mM basal 4,4'-diisothiocyanostilbene-2,2'- disulfonic acid or the removal of HCO3 from both the apical and basal baths. The rate of the exchange is reduced by acidification and increased by alkalinization. Increasing apical bath K concentration ([K]o) from 2 to 5 mM approximates the [K]o change in the subretinal space of the intact eye following a transition from light to dark. This [K]o change alkalinized the cells by increasing the rate of the apical membrane Na-HCO3 cotransporter. In 5 mM apical [K]o, the initial rate of the 0 Cl-induced alkalinization was significantly increased to 304 +/- 13% (n = 4) of control (2 mM [K]o). These mechanisms regulate pHi and could also buffer changes in subretinal pH.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antiporters,
http://linkedlifedata.com/resource/pubmed/chemical/Bicarbonates,
http://linkedlifedata.com/resource/pubmed/chemical/Chloride-Bicarbonate Antiporters,
http://linkedlifedata.com/resource/pubmed/chemical/Ions,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium
|
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
0002-9513
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
266
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
C935-45
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:8178966-Animals,
pubmed-meshheading:8178966-Antiporters,
pubmed-meshheading:8178966-Bicarbonates,
pubmed-meshheading:8178966-Cell Membrane,
pubmed-meshheading:8178966-Chloride-Bicarbonate Antiporters,
pubmed-meshheading:8178966-Female,
pubmed-meshheading:8178966-Hydrogen-Ion Concentration,
pubmed-meshheading:8178966-Intracellular Membranes,
pubmed-meshheading:8178966-Ions,
pubmed-meshheading:8178966-Male,
pubmed-meshheading:8178966-Pigment Epithelium of Eye,
pubmed-meshheading:8178966-Potassium,
pubmed-meshheading:8178966-Rana catesbeiana,
pubmed-meshheading:8178966-Sodium
|
pubmed:year |
1994
|
pubmed:articleTitle |
pHi-dependent Cl-HCO3 exchange at the basolateral membrane of frog retinal pigment epithelium.
|
pubmed:affiliation |
School of Optometry, University of California, Berkeley 94720.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|