Linked Life Data

8178437

Source:http://linkedlifedata.com/resource/pubmed/id/8178437

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-6-6
pubmed:databankReference
pubmed:abstractText
The nucleotide sequences of the glycoprotein B (gB) genes of Marek's disease virus (MDV) serotypes 2 and 3 were determined (gB-2 and gB-3, respectively). The genomic locations of these genes coincide with that of the gB gene of serotype 1 MDV (gB-1). Alignment with gB-1 (Ross et al., 1989, J. Gen. Virol. 70, 1789-1804) revealed predicted amino acid identities of 83 and 82% for gB-2 and gB-3, respectively. Excluding the predicted N-terminal signal sequences, 8 of 9 potential N-linked glycosylation sites and all 10 cysteine residues in gB-1 are conserved in both gB-2 and gB-3. In addition, the putative proteolytic cleavage sites for processing of precursors (gp100s) to gp60s and gp49s are conserved among the three gB homologs. Fowlpox virus (FPV) recombinants expressing either the gB-2 or the gB-3 gene were constructed. We detected expression of authentic gB-2 and gB-3 complexes in cells infected with these FPV recombinants. Digestion of immunoprecipitated gB-1 and gB-3 with endoglycosidases revealed that both gp60s are modified by the additions of O-glycans and complex carbohydrates after cleavage of gp100s, while gp100s and gp49s contain only high-mannose carbohydrates. We confirm that the size differences between gB-1 and gB-3 complexes are due to different carbohydrate modifications.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
200
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
484-93
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8178437-Amino Acid Sequence, pubmed-meshheading:8178437-Antigens, Viral, pubmed-meshheading:8178437-Base Sequence, pubmed-meshheading:8178437-Carbohydrate Metabolism, pubmed-meshheading:8178437-Fowlpox virus, pubmed-meshheading:8178437-Gene Expression, pubmed-meshheading:8178437-Genes, Viral, pubmed-meshheading:8178437-Glycoside Hydrolases, pubmed-meshheading:8178437-Herpesvirus 2, Gallid, pubmed-meshheading:8178437-Molecular Sequence Data, pubmed-meshheading:8178437-Polysaccharides, pubmed-meshheading:8178437-Recombinant Proteins, pubmed-meshheading:8178437-Sequence Analysis, DNA, pubmed-meshheading:8178437-Sequence Homology, Amino Acid, pubmed-meshheading:8178437-Sequence Homology, Nucleic Acid, pubmed-meshheading:8178437-Serotyping, pubmed-meshheading:8178437-Viral Envelope Proteins
pubmed:year
1994
pubmed:articleTitle
The glycoprotein B genes of Marek's disease virus serotypes 2 and 3: identification and expression by recombinant fowlpox viruses.
pubmed:affiliation
USDA-Agricultural Research Service, Avian Disease and Oncology Laboratory, East Lansing, Michigan 48823.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't