Linked Life Data

8176428

Source:http://linkedlifedata.com/resource/pubmed/id/8176428

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-6-9
pubmed:abstractText
1. The effect of intracellular application of inositol 1,4,5-trisphosphate (IP3) from the patch pipette was analyzed in isolated rat olfactory neurons under whole-cell patch clamp. 2. Intracellular dialysis of 10 microM 1,4,5-IP3 in K(+)-internal solution induced a sustained depolarization of 35.8 +/- 10.5 (SD) mV (n = 16). The IP3-induced response was observed in 75% of the cells dialyzed with IP3 but not when 10 microM ruthenium red was also included in the pipette solution (4 cells). Lower concentrations (50-100 nM) of 2,4,5-IP3 induced similar responses to those produced by 1,4,5-IP3 in five of eight olfactory neurons. 3. Steady-state I-V relationships of IP3-gated currents with K(+)-internal solution were classified into two types: outwardly rectifying and N-shaped. In Cs(+)-internal solution outwardly rectifying and linear patterns were observed. 4. The IP3-induced currents were inhibited by external Cd2+ (1 mM). The reversal potentials of the Cd(2+)-inhibitable currents were -16.1 mV (n = 2) and -29.0 +/- 7.1 mV (n = 3) for the outwardly rectifying and N-shaped types, respectively, in K(+)-internal solution. The reversal potential was -5.9 +/- 6.8 mV (n = 5) in the Cs(+)-internal solution. 6. In contrast, the Ca(2+)-ionophore, ionomycin (5 microM) hyperpolarized the olfactory neurons and greatly potentiated the outward currents at positive holding membrane potential. 7. The data suggest that IP3 can depolarize rat olfactory neurons without mediation by intracellular Ca2+.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-3077
pubmed:author
pubmed:issnType
Print
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
595-602
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Inositol 1,4,5-trisphosphate-gated conductance in isolated rat olfactory neurons.
pubmed:affiliation
Monell Chemical Senses Center, Philadelphia, Pennsylvania.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.