Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
1994-6-3
|
pubmed:abstractText |
Flow cytometry and two-colour immunofluorescence were used to detect cytoplasmic bovine viral diarrhoea virus (BVDV) antigen in leukocytes from viraemic cattle. Monoclonal antibody to the p80 protein of BVDV, a non-structural viral antigen, was used to identify the subpopulations of leukocytes in which viral protein synthesis had occurred. Viral antigen was detected in 23% of peripheral blood mononuclear cells. Monocytes were found to have the highest frequency of infection (35%). A higher proportion of CD2+ T cells (23%) were infected, compared with B cells (11%) or WC1+ gamma delta T cells (11%). No significant differences in percentages of different leukocyte subpopulations in blood were detected in persistently viraemic animals compared with controls.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
May
|
pubmed:issn |
0022-1317
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
75 ( Pt 5)
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1189-94
|
pubmed:dateRevised |
2003-11-14
|
pubmed:meshHeading |
pubmed-meshheading:8176381-Animals,
pubmed-meshheading:8176381-Antibodies, Monoclonal,
pubmed-meshheading:8176381-Antibodies, Viral,
pubmed-meshheading:8176381-Antigens, Viral,
pubmed-meshheading:8176381-Bovine Virus Diarrhea-Mucosal Disease,
pubmed-meshheading:8176381-Cattle,
pubmed-meshheading:8176381-Flow Cytometry,
pubmed-meshheading:8176381-Leukocytes,
pubmed-meshheading:8176381-Monocytes,
pubmed-meshheading:8176381-Viral Nonstructural Proteins,
pubmed-meshheading:8176381-Viremia
|
pubmed:year |
1994
|
pubmed:articleTitle |
Detection of bovine viral diarrhoea virus p80 protein in subpopulations of bovine leukocytes.
|
pubmed:affiliation |
AFRC Institute for Animal Health, Compton Laboratory, Newbury, Berkshire, U.K.
|
pubmed:publicationType |
Journal Article
|