|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1994-6-3
|
|
pubmed:abstractText |
Lectin affinity chromatography was used to demonstrate the glycoprotein nature of the serotonin (5-HT) transporter. The human platelet transporter protein was solubilized with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), labelled with [3H]cyanoimipramine and chromatographed on lectin columns. Wheat germ, gorse seed and lentil lectin-agarose columns specifically retained 5-HT transporter. Peanut and horse gram lectins were ineffective. Concanavalin A showed high non-specific adsorption. Binding of [3H]imipramine to platelet membranes or solubilized 5-HT transporter was not affected by lectins. These data suggest that the lectin interaction with 5-HT transporter is extrinsic to the antidepressant binding site.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3-((3-cholamidopropyl)dimethylammoni...,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cholic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Detergents,
http://linkedlifedata.com/resource/pubmed/chemical/Imipramine,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SLC6A4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin Plasma Membrane...
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0014-2999
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
266
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
327-31
|
|
pubmed:dateRevised |
2005-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:8174615-Binding Sites,
pubmed-meshheading:8174615-Blood Platelets,
pubmed-meshheading:8174615-Carrier Proteins,
pubmed-meshheading:8174615-Cholic Acids,
pubmed-meshheading:8174615-Chromatography, Affinity,
pubmed-meshheading:8174615-Detergents,
pubmed-meshheading:8174615-Humans,
pubmed-meshheading:8174615-Imipramine,
pubmed-meshheading:8174615-Lectins,
pubmed-meshheading:8174615-Membrane Glycoproteins,
pubmed-meshheading:8174615-Membrane Transport Proteins,
pubmed-meshheading:8174615-Nerve Tissue Proteins,
pubmed-meshheading:8174615-Serotonin,
pubmed-meshheading:8174615-Serotonin Plasma Membrane Transport Proteins
|
|
pubmed:year |
1994
|
|
pubmed:articleTitle |
Interaction of lectins with human platelet serotonin transporter.
|
|
pubmed:affiliation |
Veterans Administration Medical Center, Long Beach, CA 90822.
|
|
pubmed:publicationType |
Journal Article
|
