Linked Life Data

8174551

Source:http://linkedlifedata.com/resource/pubmed/id/8174551

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-6-9
pubmed:abstractText
Photosystem II membrane fractions from dark-adapted mesophyll chloroplasts of maize were solubilized in different concentrations of dodecyl beta-D-maltoside. Chlorophyll-binding proteins from photosystem II were isolated either by ultracentrifugation on a sucrose gradient, or by flat bed isoelectric focusing and identified by gel electrophoresis analysis for their polypeptide composition. Lipid and fatty acid compositions were determined in complexes prepared by both methods and also in purified light-harvesting complex II, in minor chlorophyll a/b binding complexes 29, 26, 24, in photosystem II antennae (chlorophyll-protein complexes 43, 47) and in the photosystem II reaction centers chlorophyll-protein complexes. Comparative analysis of the results suggests that a true heterogeneity exists in the lipid class distribution among the different chlorophyll-protein complexes in this region of the photosynthetic membrane. Photosystem II core fractions prepared either by ultra-centrifugation on a sucrose gradient or by isoelectric focusing were found significantly enriched in monogalactosyldiacylglycerol; fractionation of the photosystem II core in its components showed that it was the chlorophyll-protein complexes 43 and 47 which were mainly responsible for this enrichment. One of them, the chlorophyll-protein complex 47, was found containing monogalactosyldiacylglycerol and having a very high level of saturated fatty acids. The minor chlorophyll a/b binding linkers (chlorophyll-protein complexes 24, 26 and 29) retain a largely higher amount of lipids than all other complexes and especially of highly unsaturated galactolipids. Concerning the main light-harvesting antenna (LHCII), it is demonstrated that phosphatidylglycerol is strongly linked to the complex if it cannot be detached at high detergent concentration, while many galactolipids (which nevertheless represent the major lipid classes) are lost. This main light-harvesting complex has been fractionated into several families by isoelectric focusing showing a marked difference in lipid and polypeptide composition. A spectacular increase in the phosphatidylglycerol content was observed in the fraction migrating near the anode and enriched in a 26-kDa polypeptide; but this result is difficult to interpret in physiological terms as it was shown that phosphatidylglycerol alone, because of its negative charge, also migrates toward the anode in isoelectric focusing.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
221
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
721-30
pubmed:dateRevised
2007-7-23
pubmed:meshHeading
pubmed-meshheading:8174551-Chemical Fractionation, pubmed-meshheading:8174551-Chlorophyll, pubmed-meshheading:8174551-Chloroplasts, pubmed-meshheading:8174551-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:8174551-Fatty Acids, pubmed-meshheading:8174551-Glucosides, pubmed-meshheading:8174551-Immunoblotting, pubmed-meshheading:8174551-Isoelectric Focusing, pubmed-meshheading:8174551-Light-Harvesting Protein Complexes, pubmed-meshheading:8174551-Lipids, pubmed-meshheading:8174551-Molecular Weight, pubmed-meshheading:8174551-Phosphatidylglycerols, pubmed-meshheading:8174551-Photosynthetic Reaction Center Complex Proteins, pubmed-meshheading:8174551-Photosystem II Protein Complex, pubmed-meshheading:8174551-Solubility, pubmed-meshheading:8174551-Ultracentrifugation, pubmed-meshheading:8174551-Zea mays
pubmed:year
1994
pubmed:articleTitle
Heterogenous lipid distribution among chlorophyll-binding proteins of photosystem II in maize mesophyll chloroplasts.
pubmed:affiliation
Laboratoire de Biochimie Fonctionnelle des Membranes Végétales, URA 1128, CNRS, Gif sur Yvette, France.
pubmed:publicationType
Journal Article, Comparative Study