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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1994-6-9
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pubmed:abstractText |
The role of carbonic anhydrase in the process of proximal duodenal mucosal bicarbonate secretion was investigated in the guinea pig. In a series of experiments in vivo, the duodenum was perfused with 24 mmol/liter NaHCO3 solution (+ NaCl for isotonicity) to ensure that active duodenal HCO3- secretion against a concentration gradient was measured. Acetazolamide (80 mg/kg) was infused intravenously to examine the role of carbonic anhydrase on basal and agonist-stimulated HCO3- secretion. Acetazolamide abolished basal HCO3- secretion and significantly decreased HCO3- secretion after stimulation with dibutyryl 5'-cyclic adenosine monophosphate (dBcAMP, 10(-5) mol/kg), dibutyryl 5'-cyclic guanosine monophosphate (dBcGMP, 10(-5) mol/kg), prostaglandin E2 (PGE2, 10(-6) mol/kg), PGF2 alpha (10(-6) mol/kg), tetradecanoyl-phorbol-acetate (TPA, 10(-7) mol/kg), glucagon (10(-7) mol/kg), vasoactive intestinal polypeptide (VIP, 10(-8) mol/kg), and carbachol (10(-8) mol/kg). Utilizing a fluorescence technique, we could detect the enzyme carbonic anhydrase in equal amounts in villous and crypt cells of the proximal duodenal epithelium; no activity was demonstrated in tissues pretreated with acetazolamide. In conclusion, carbonic anhydrase is required for both basal and stimulated duodenal HCO3- secretion.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetazolamide,
http://linkedlifedata.com/resource/pubmed/chemical/Bicarbonates,
http://linkedlifedata.com/resource/pubmed/chemical/Bucladesine,
http://linkedlifedata.com/resource/pubmed/chemical/Carbachol,
http://linkedlifedata.com/resource/pubmed/chemical/Carbonic Anhydrases,
http://linkedlifedata.com/resource/pubmed/chemical/Dibutyryl Cyclic GMP,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Glucagon,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/Vasoactive Intestinal Peptide
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0163-2116
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1078-84
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8174420-Acetazolamide,
pubmed-meshheading:8174420-Animals,
pubmed-meshheading:8174420-Bicarbonates,
pubmed-meshheading:8174420-Bucladesine,
pubmed-meshheading:8174420-Carbachol,
pubmed-meshheading:8174420-Carbonic Anhydrases,
pubmed-meshheading:8174420-Dibutyryl Cyclic GMP,
pubmed-meshheading:8174420-Dinoprostone,
pubmed-meshheading:8174420-Duodenum,
pubmed-meshheading:8174420-Glucagon,
pubmed-meshheading:8174420-Guinea Pigs,
pubmed-meshheading:8174420-Male,
pubmed-meshheading:8174420-Tetradecanoylphorbol Acetate,
pubmed-meshheading:8174420-Vasoactive Intestinal Peptide
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pubmed:year |
1994
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pubmed:articleTitle |
Role of carbonic anhydrase in basal and stimulated bicarbonate secretion by the guinea pig duodenum.
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pubmed:affiliation |
Gastroenterology Institute, Soroka Medical Center, Beer Sheva, Israel.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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