Linked Life Data

8174219

Source:http://linkedlifedata.com/resource/pubmed/id/8174219

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-6-7
pubmed:abstractText
According to known SSUrDNA sequences of Plasmodium vivax, correlated protozoa and human being, sequences of oligonucleotide primers were defined with computer programming. Specific SSUrDNA fragment of P. vivax, about 640 bp in length, was directly amplified by two temperature point polymerase chain reaction from extracted genomic DNA of two blood samples of vivax malaria patients from Yunnan Province. Using dideoxynucleotide terminator method, the sequences of amplified DNA fragments were determined separately and showed no difference between the two samples. However, comparison of the sequence reported by Waters AP and McCutchan TF (1989) and that of amplified fragment of Yunnan P. vivax isolates revealed the existence of nucleotide substitution and deletion which occurred respectively in the sites 269 and 630, and resulted in the change of restriction map.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1000-7423
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
81-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
[Amplification, cloning and sequence analysis of a SSUrRNA gene fragment of Plasmodium vivax isolates from Yunnan Province].
pubmed:affiliation
Department of Parasitology, Fourth Military Medical University, Xian Shaanxi.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't