| pubmed-article:8174132 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8174132 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:8174132 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:8174132 | lifeskim:mentions | umls-concept:C0969709 | lld:lifeskim |
| pubmed-article:8174132 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:8174132 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:8174132 | pubmed:dateCreated | 1994-6-6 | lld:pubmed |
| pubmed-article:8174132 | pubmed:abstractText | The present work has examined the effects of okadaic acid, an inhibitor of serine/threonine protein phosphatase, PP-1 and PP-2A, on the regulation of EGR-1 gene expression in normal peripheral blood T- and Jurkat cells. The results demonstrate that okadaic acid treatment is associated with a transient induction of EGR-1 gene expression which was detectable by 30 min to 1 h and peaked at 3-6 h. EGR-1 mRNA was superinduced in cells treated with both okadaic acid and the protein synthesis inhibitor cycloheximide. The half-life of EGR-1 mRNA was similar in both control and okadaic acid-treated cells. In contrast, treatment with both okadaic acid and cycloheximide prolonged the half-life of EGR-1 transcripts. Nuclear run-on assays demonstrated that induction of EGR-1 gene expression by okadaic acid is controlled at least in part by a transcriptional mechanism. Transient expression assays with EGR-1 promotor fragments linked to the chloramphenicol acetyltransferase gene demonstrate that okadaic acid-induced EGR-1 transcription is conferred by the 5' most distal CArG box, CC (AT)6GG, in the EGR-1 promoter. Moreover, chloramphenicol acetyltransferase activity was induced by okadaic acid when the 5' most distal CArG element was linked to the heterologous herpes simplex virus thymidine kinase promoter, and not induced with a similar heterologous construct containing a mutated CArG sequence. These studies demonstrate that okadaic acid regulates EGR-1 gene expression at the transcriptional level via the CArG element and suggest that PP-1 and PP-2A play a role in T-cell activation. | lld:pubmed |
| pubmed-article:8174132 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8174132 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8174132 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8174132 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8174132 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8174132 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8174132 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8174132 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8174132 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8174132 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8174132 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8174132 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8174132 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8174132 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8174132 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8174132 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8174132 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8174132 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:8174132 | pubmed:issn | 0008-5472 | lld:pubmed |
| pubmed-article:8174132 | pubmed:author | pubmed-author:UchiyamaHH | lld:pubmed |
| pubmed-article:8174132 | pubmed:author | pubmed-author:KufeD WDW | lld:pubmed |
| pubmed-article:8174132 | pubmed:author | pubmed-author:AndersonK CKC | lld:pubmed |
| pubmed-article:8174132 | pubmed:author | pubmed-author:SukhatmeV PVP | lld:pubmed |
| pubmed-article:8174132 | pubmed:author | pubmed-author:ChauhanDD | lld:pubmed |
| pubmed-article:8174132 | pubmed:author | pubmed-author:KharbandaS... | lld:pubmed |
| pubmed-article:8174132 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8174132 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:8174132 | pubmed:volume | 54 | lld:pubmed |
| pubmed-article:8174132 | pubmed:geneSymbol | c-fos | lld:pubmed |
| pubmed-article:8174132 | pubmed:geneSymbol | c-myc | lld:pubmed |
| pubmed-article:8174132 | pubmed:geneSymbol | c-jun | lld:pubmed |
| pubmed-article:8174132 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8174132 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8174132 | pubmed:pagination | 2234-9 | lld:pubmed |
| pubmed-article:8174132 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:8174132 | pubmed:meshHeading | pubmed-meshheading:8174132-... | lld:pubmed |
| pubmed-article:8174132 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8174132 | pubmed:articleTitle | Involvement of serum response element in okadaic acid-induced EGR-1 transcription in human T-cells. | lld:pubmed |
| pubmed-article:8174132 | pubmed:affiliation | Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115. | lld:pubmed |
| pubmed-article:8174132 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8174132 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8174132 | lld:pubmed |
