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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1994-6-6
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pubmed:abstractText |
The present work has examined the effects of okadaic acid, an inhibitor of serine/threonine protein phosphatase, PP-1 and PP-2A, on the regulation of EGR-1 gene expression in normal peripheral blood T- and Jurkat cells. The results demonstrate that okadaic acid treatment is associated with a transient induction of EGR-1 gene expression which was detectable by 30 min to 1 h and peaked at 3-6 h. EGR-1 mRNA was superinduced in cells treated with both okadaic acid and the protein synthesis inhibitor cycloheximide. The half-life of EGR-1 mRNA was similar in both control and okadaic acid-treated cells. In contrast, treatment with both okadaic acid and cycloheximide prolonged the half-life of EGR-1 transcripts. Nuclear run-on assays demonstrated that induction of EGR-1 gene expression by okadaic acid is controlled at least in part by a transcriptional mechanism. Transient expression assays with EGR-1 promotor fragments linked to the chloramphenicol acetyltransferase gene demonstrate that okadaic acid-induced EGR-1 transcription is conferred by the 5' most distal CArG box, CC (AT)6GG, in the EGR-1 promoter. Moreover, chloramphenicol acetyltransferase activity was induced by okadaic acid when the 5' most distal CArG element was linked to the heterologous herpes simplex virus thymidine kinase promoter, and not induced with a similar heterologous construct containing a mutated CArG sequence. These studies demonstrate that okadaic acid regulates EGR-1 gene expression at the transcriptional level via the CArG element and suggest that PP-1 and PP-2A play a role in T-cell activation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/EGR1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Early Growth Response Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/Ethers, Cyclic,
http://linkedlifedata.com/resource/pubmed/chemical/Immediate-Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Okadaic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0008-5472
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
54
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pubmed:geneSymbol |
c-fos,
c-jun,
c-myc
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2234-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8174132-Base Sequence,
pubmed-meshheading:8174132-Cell Line,
pubmed-meshheading:8174132-Cell Nucleus,
pubmed-meshheading:8174132-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:8174132-Cycloheximide,
pubmed-meshheading:8174132-DNA-Binding Proteins,
pubmed-meshheading:8174132-Early Growth Response Protein 1,
pubmed-meshheading:8174132-Ethers, Cyclic,
pubmed-meshheading:8174132-Gene Expression,
pubmed-meshheading:8174132-Humans,
pubmed-meshheading:8174132-Immediate-Early Proteins,
pubmed-meshheading:8174132-Molecular Sequence Data,
pubmed-meshheading:8174132-Okadaic Acid,
pubmed-meshheading:8174132-Promoter Regions, Genetic,
pubmed-meshheading:8174132-Protein Tyrosine Phosphatases,
pubmed-meshheading:8174132-RNA, Messenger,
pubmed-meshheading:8174132-Restriction Mapping,
pubmed-meshheading:8174132-T-Lymphocytes,
pubmed-meshheading:8174132-Transcription, Genetic,
pubmed-meshheading:8174132-Transcription Factors,
pubmed-meshheading:8174132-Transfection,
pubmed-meshheading:8174132-Tumor Cells, Cultured,
pubmed-meshheading:8174132-Zinc Fingers
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pubmed:year |
1994
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pubmed:articleTitle |
Involvement of serum response element in okadaic acid-induced EGR-1 transcription in human T-cells.
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pubmed:affiliation |
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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