Linked Life Data

8173800

Source:http://linkedlifedata.com/resource/pubmed/id/8173800

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1994-6-9
pubmed:abstractText
We have examined the targeting of S. typhimurium-containing vacuoles to lysosomes after invasion of cultured HeLa epithelial cells. Our results show that intracellular bacteria colocalize with vacuoles containing lysosomal membrane glycoproteins (LGPs). Both human LGPs, hlamp-1 and hlamp-2, are present in S. typhimurium-containing vacuoles from approximately 2 h postinfection. At later times (4-6 h), long and stable filamentous structures with lysosomal markers appear connected to bacteria-containing vacuoles in infected cells. Viable intracellular bacteria are required for the formation of these structures, which are not detected in uninfected cells or in HeLa epithelial cells infected with another invasive bacteria, Yersinia enterocolitica. Kinetics analysis showed a strict correlation between the appearance of these LGP-rich filaments and the initiation of intracellular bacterial replication. Moreover, these structures are absent in epithelial cells infected with certain S. typhimurium intracellular replication-defective mutants. Additional data confirmed that an intact microtubule network and intravacuolar acidic pH are required to induce the formation of LGP-containing filamentous structures and that these structures are morphologically and functionally different from previously described tubular lysosomes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1056-2044
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
227-31
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Intracellular replication of Salmonella within epithelial cells is associated with filamentous structures containing lysosomal membrane glycoproteins.
pubmed:affiliation
Biotechnology Laboratory, University of British Columbia, Vancouver, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't